C57BL/6JCya-Slc22a18em1flox/Cya
Common Name:
Slc22a18-flox
Product ID:
S-CKO-04125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc22a18-flox
Strain ID
CKOCMP-18400-Slc22a18-B6J-VA
Gene Name
Product ID
S-CKO-04125
Gene Alias
BWR1A; BWSCR1A; HET; ITM; Impt1; Orctl2; Slc22a1l; TSSC5; p45-BWR1A
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc22a18em1flox/Cya mice (Catalog S-CKO-04125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052348
NCBI RefSeq
NM_008767
Target Region
Exon 3~5
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Slc22a18, solute carrier family 22 member 18, is an orphan transporter whose endogenous substrate remains unknown. It has been associated with lipid metabolism and tumor-related processes. In lipid metabolism, it may be involved in pathways regulating triglyceride synthesis and degradation. In tumors, its chromosomal location at 11p15.5 and aberrant expression in various cancers suggest it could be a tumor-related gene, potentially acting as a tumor suppressor [4,5,6,7,8,9]. Genetic models, such as knockout mice, are valuable for studying its functions.
Slc22a18 knockout mice grew normally but showed decreased hepatic triglyceride content under refeeding conditions, reduced epididymal fat mass, and lower liver weight with leptin deficiency, indicating its positive role in lipid accumulation in vivo [1]. In HepG2 cells, knockdown of SLC22A18 impaired lipid metabolism, suppressed lipid degradation, and increased cell invasiveness [3]. In colorectal cancer, SLC22A18 knockdown led to oxaliplatin resistance, and its low expression was correlated with worse survival, with ERK activation involved in this resistance mechanism [2]. Missense variants of SLC22A18 in colon cancer cells led to higher cell proliferation, migration, and invasion compared to the wild-type [7].
In conclusion, Slc22a18 plays a significant role in lipid metabolism, promoting fat accumulation in vivo. In the context of diseases, it shows potential as a biomarker and is involved in tumor-related processes such as tumor suppression, cell invasion, and drug resistance. Studies using knockout mouse models and cell-based knockdown experiments have been crucial in revealing these functions, providing insights into the underlying biological mechanisms and potential therapeutic targets for lipid-related disorders and cancers.
References:
1. Yamamoto, Takashi, Iizuka, Yoko, Izumi-Yamamoto, Kozue, Fujita, Toshiro, Gotoda, Takanari. 2024. Overexpression of Slc22a18 facilitates fat accumulation in mice. In Biochemical and biophysical research communications, 712-713, 149922. doi:10.1016/j.bbrc.2024.149922. https://pubmed.ncbi.nlm.nih.gov/38626531/
2. Kim, Tae Won, Pyo, Dae Hee, Ko, Eunbyeol, Lee, Woo Yong, Cho, Yong Beom. 2022. Expression of SLC22A18 regulates oxaliplatin resistance by modulating the ERK pathway in colorectal cancer. In American journal of cancer research, 12, 1393-1408. doi:. https://pubmed.ncbi.nlm.nih.gov/35411243/
3. Ito, Shingo, Honda, Gentaro, Fujino, Yu, Hirayama-Kurogi, Mio, Ohtsuki, Sumio. 2019. Knockdown of Orphan Transporter SLC22A18 Impairs Lipid Metabolism and Increases Invasiveness of HepG2 Cells. In Pharmaceutical research, 36, 39. doi:10.1007/s11095-018-2565-4. https://pubmed.ncbi.nlm.nih.gov/30635741/
4. Jung, Yeonjoo, Jun, Yukyung, Lee, Hee-Young, Lee, Sanghyuk, Kim, Jaesang. . Characterization of SLC22A18 as a tumor suppressor and novel biomarker in colorectal cancer. In Oncotarget, 6, 25368-80. doi:10.18632/oncotarget.4681. https://pubmed.ncbi.nlm.nih.gov/26196590/
5. Chu, Sheng-Hua, Ma, Yan-Bin, Feng, Dong-Fu, Li, Zhi-Qiang, Jiang, Pu-Cha. 2011. Correlation of low SLC22A18 expression with poor prognosis in patients with glioma. In Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, 19, 95-8. doi:10.1016/j.jocn.2011.04.032. https://pubmed.ncbi.nlm.nih.gov/22153794/
6. Ito, Shingo, Fujino, Yu, Ogata, Seiryo, Hirayama-Kurogi, Mio, Ohtsuki, Sumio. 2018. Involvement of an Orphan Transporter, SLC22A18, in Cell Growth and Drug Resistance of Human Breast Cancer MCF7 Cells. In Journal of pharmaceutical sciences, 107, 3163-3170. doi:10.1016/j.xphs.2018.08.011. https://pubmed.ncbi.nlm.nih.gov/30145211/
7. Song, Hyo Sook, Ha, Seung Yeon, Kim, Jin-Young, Kim, Minsuk, Choi, Ji Ha. 2024. The effect of genetic variants of SLC22A18 on proliferation, migration, and invasion of colon cancer cells. In Scientific reports, 14, 3925. doi:10.1038/s41598-024-54658-w. https://pubmed.ncbi.nlm.nih.gov/38366023/
8. He, Hongyu, Xu, Cheng, Zhao, Ziqin, Xu, Hongmei, Zhang, Hongwei. 2010. Low expression of SLC22A18 predicts poor survival outcome in patients with breast cancer after surgery. In Cancer epidemiology, 35, 279-85. doi:10.1016/j.canep.2010.09.006. https://pubmed.ncbi.nlm.nih.gov/21144813/
9. Chu, Sheng-Hua, Feng, Dong-Fu, Ma, Yan-Bin, Li, Zhi-Qiang, Jiang, Pu-Cha. 2011. Promoter methylation and downregulation of SLC22A18 are associated with the development and progression of human glioma. In Journal of translational medicine, 9, 156. doi:10.1186/1479-5876-9-156. https://pubmed.ncbi.nlm.nih.gov/21936894/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen