C57BL/6NCya-Pdcd1em1flox/Cya
Common Name:
Pdcd1-flox
Product ID:
S-CKO-04199
Background:
C57BL/6NCya
Product Type
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Basic Information
Strain Name
Pdcd1-flox
Strain ID
CKOCMP-18566-Pdcd1-B6N-VA
Gene Name
Product ID
S-CKO-04199
Gene Alias
Ly101; PD-1; Pdc1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pdcd1em1flox/Cya mice (Catalog S-CKO-04199) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027507
NCBI RefSeq
NM_008798
Target Region
Exon 2~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Pdcd1, encoding PD-1, is an important gene as PD-1 is a key immune checkpoint protein and a major immunotherapy target for treating cancer [1,2]. It is involved in immune-related pathways, and its dysregulation can lead to abnormal immune responses. Alternative splicing of Pdcd1 generates an exon 3-skipped isoform PD-1Δ3, which is suggested to have an antagonistic role to PD-1 [1].
The mechanism underlying Pdcd1 exon 3 splicing has been explored. Through a minigene system, exon 3 skipping was confirmed as the main alternative splicing event. Two splicing enhancers (ESE3a and ESE3b) in exon 3 were mapped by deletion analysis. MATR3 was defined as a splicing activator during Pdcd1 exon 3 splicing through binding to ESE3b, and its activity is counteracted by an RNA secondary structure around ESE3b and an RNA helicase DDX5. ASOs that efficiently promote Pdcd1 exon 3 skipping in both minigene and endogenous-gene contexts were also identified [1].
In conclusion, Pdcd1 is crucial in cancer immunotherapy due to its role in encoding the key immune checkpoint protein PD-1. Understanding the splicing mechanism of Pdcd1 provides insights into potential drug development, as the identified ASOs may be further studied as potential drug candidates for cancer treatment [1].
References:
1. Sun, Junjie, Bai, Jialin, Jiang, Tao, Gao, Yuan, Hua, Yimin. 2019. Modulation of PDCD1 exon 3 splicing. In RNA biology, 16, 1794-1805. doi:10.1080/15476286.2019.1659080. https://pubmed.ncbi.nlm.nih.gov/31441370/
2. Mishra, Alok, Verma, Mukesh. . Epigenetic and Genetic Regulation of PDCD1 Gene in Cancer Immunology. In Methods in molecular biology (Clifton, N.J.), 1856, 247-254. doi:10.1007/978-1-4939-8751-1_14. https://pubmed.ncbi.nlm.nih.gov/30178256/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen