C57BL/6JCya-Ppp1r17em1flox/Cya
Common Name:
Ppp1r17-flox
Product ID:
S-CKO-04415
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppp1r17-flox
Strain ID
CKOCMP-19051-Ppp1r17-B6J-VA
Gene Name
Product ID
S-CKO-04415
Gene Alias
Gsbs; Ppp1r2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1r17em1flox/Cya mice (Catalog S-CKO-04415) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052827
NCBI RefSeq
NM_011153
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ppp1r17, the protein phosphatase 1 regulatory subunit 17, is involved in multiple biological processes. It has been associated with the regulation of synaptic function, with its phosphorylation and nuclear-cytoplasmic translocation affecting gene expression in this regard [1]. It may also be related to neural progenitor cell cycle progression as it has been shown to slow down this process, paralleling changes seen in primate and human neurodevelopment [3].
In mice, DMH-specific Prkg1 knockdown, which suppresses age-associated Ppp1r17 translocation, and the chemogenetic activation of DMHPpp1r17 neurons significantly ameliorate age-associated dysfunction in white adipose tissue (WAT), increase physical activity, and extend lifespan. This indicates that DMHPpp1r17 neurons marked by Ppp1r17 expression regulate aging and longevity in mice through hypothalamic-adipose inter-tissue communication [1]. Also, in ob/ob mice, chemogenetic activation of PPP1R17 neurons decreased food intake and body weight, while inhibition increased them, suggesting that these neurons function to restrict food intake to limit overconsumption [2].
In conclusion, Ppp1r17 plays a crucial role in regulating aging, lifespan, and food intake in mice. The studies using mouse models have revealed its significance in hypothalamic-adipose inter-tissue communication and the control of food intake, providing insights into potential mechanisms related to age-associated diseases and metabolic disorders.
References:
1. Tokizane, Kyohei, Brace, Cynthia S, Imai, Shin-Ichiro. 2024. DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. In Cell metabolism, 36, 377-392.e11. doi:10.1016/j.cmet.2023.12.011. https://pubmed.ncbi.nlm.nih.gov/38194970/
2. Caglar, Caner, Friedman, Jeffrey. . Restriction of food intake by PPP1R17-expressing neurons in the DMH. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2100194118. https://pubmed.ncbi.nlm.nih.gov/33753517/
3. Girskis, Kelly M, Stergachis, Andrew B, DeGennaro, Ellen M, Greenberg, Michael E, Walsh, Christopher A. 2021. Rewiring of human neurodevelopmental gene regulatory programs by human accelerated regions. In Neuron, 109, 3239-3251.e7. doi:10.1016/j.neuron.2021.08.005. https://pubmed.ncbi.nlm.nih.gov/34478631/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen