C57BL/6NCya-Ppp3cbem1flox/Cya
Common Name
Ppp3cb-flox
Product ID
S-CKO-04419
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-19056-Ppp3cb-B6N-VA
When using this mouse strain in a publication, please cite “Ppp3cb-flox Mouse (Catalog S-CKO-04419) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ppp3cb-flox
Strain ID
CKOCMP-19056-Ppp3cb-B6N-VA
Gene Name
Product ID
S-CKO-04419
Gene Alias
1110063J16Rik, Calnb, CnAbeta, Cnab
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000159027
NCBI RefSeq
NM_008914
Target Region
Exon 2~3
Size of Effective Region
~1.4 kb
Overview of Gene Research
Ppp3cb, encoding the catalytic subunit 2B of calcineurin, is a key component of the serine/threonine phosphatase calcineurin. It dephosphorylates substrate proteins, thereby regulating their physiological activities and is involved in multiple pathways such as the calcineurin/MEK/ERK signaling pathway. It plays an important role in various biological processes and diseases [1,2,3,4,5,6,7,8,9,10].
In a mouse model lacking Ppp3cb (PPP3CB KO), upon AngII treatment, these mice showed less aortic media thickening, lumen dilation, and systolic blood pressure compared to wild-type mice. This indicates that Ppp3cb is involved in AngII-induced vascular remodelling, which may occur via enhancing heparin-binding EGF-like growth factor (HB-EGF) secretion, epidermal growth factor receptor (EGFR) activation, and consecutive stimulation of transforming growth factor β (TGFβ) and connective tissue growth factor (CTGF) signalling [4].
In conclusion, Ppp3cb is crucial for multiple biological processes. Its role in diseases like non-small-cell lung cancer (NSCLC), neuroblastoma, and liver injury has been identified through various studies. The PPP3CB KO mouse model has significantly contributed to understanding its role in AngII-dependent vascular remodelling, providing insights into related disease mechanisms and potential therapeutic targets.
References：
1. Gazzeri, Sylvie, Zubchuk, Nadiia, Montaudon, Elodie, Toffart, Anne-Claire, Eymin, Beatrice. 2024. PPP3CB overexpression mediates EGFR TKI resistance in lung tumors via calcineurin/MEK/ERK signaling. In Life science alliance, 7, . doi:10.26508/lsa.202402873. https://pubmed.ncbi.nlm.nih.gov/39353739/
2. Shakhova, Irina, Li, Yuanyuan, Yu, Fan, Rumyantsev, Alexander G, Nakagawara, Akira. 2018. PPP3CB contributes to poor prognosis through activating nuclear factor of activated T-cells signaling in neuroblastoma. In Molecular carcinogenesis, 58, 426-435. doi:10.1002/mc.22939. https://pubmed.ncbi.nlm.nih.gov/30457174/
3. Fang, Zhiyuan, Xu, Yanyong, Liu, Guowen, Du, Xiliang, Li, Xinwei. 2023. Narirutin activates TFEB (transcription factor EB) to protect against Acetaminophen-induced liver injury by targeting PPP3/calcineurin. In Autophagy, 19, 2240-2256. doi:10.1080/15548627.2023.2179781. https://pubmed.ncbi.nlm.nih.gov/36779633/
4. Nolze, Alexander, Köhler, Conny, Ruhs, Stefanie, Gekle, Michael, Grossmann, Claudia. 2021. Calcineurin (PPP3CB) regulates angiotensin II-dependent vascular remodelling by potentiating EGFR signalling in mice. In Acta physiologica (Oxford, England), 233, e13715. doi:10.1111/apha.13715. https://pubmed.ncbi.nlm.nih.gov/34228904/
5. Chen, Lei, He, Qingling, Liu, Yamin, Zhou, Qin, Li, Qianyin. 2019. PPP3CB Inhibits Migration of G401 Cells via Regulating Epithelial-to-Mesenchymal Transition and Promotes G401 Cells Growth. In International journal of molecular sciences, 20, . doi:10.3390/ijms20020275. https://pubmed.ncbi.nlm.nih.gov/30641937/
6. Xia, Qin, Zheng, Hanfei, Li, Yang, Zhang, Lingqiang, Dong, Lei. 2023. SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis. In Autophagy, 20, 735-751. doi:10.1080/15548627.2023.2267413. https://pubmed.ncbi.nlm.nih.gov/37909662/
7. Rusmini, Paola, Cortese, Katia, Crippa, Valeria, Vaccari, Thomas, Poletti, Angelo. 2018. Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration. In Autophagy, 15, 631-651. doi:10.1080/15548627.2018.1535292. https://pubmed.ncbi.nlm.nih.gov/30335591/
8. Qiu, Xiangmin, Jiang, Ziqing, Luo, Yu, Song, Tao, Li, Qianyin. . PPP3CB Inhibits Cell Proliferation and the Warburg Effect in Bladder Cancer by Blocking PDHK1. In Frontiers in bioscience (Landmark edition), 29, 48. doi:10.31083/j.fbl2902048. https://pubmed.ncbi.nlm.nih.gov/38420800/
9. Xia, Qin, Liu, Xuan, Zhong, Lu, Qu, Jun, Dong, Lei. 2024. SMURF1 mediates damaged lysosomal homeostasis by ubiquitinating PPP3CB to promote the activation of TFEB. In Autophagy, 21, 530-547. doi:10.1080/15548627.2024.2407709. https://pubmed.ncbi.nlm.nih.gov/39324484/
10. Chen, Jingchen, Balakrishnan-Renuka, Ajeesh, Hagemann, Nina, Erdmann, Kai S, Brand-Saberi, Beate. 2015. A novel interaction between ATOH8 and PPP3CB. In Histochemistry and cell biology, 145, 5-16. doi:10.1007/s00418-015-1368-5. https://pubmed.ncbi.nlm.nih.gov/26496921/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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