C57BL/6JCya-Psmd4em1flox/Cya
Common Name:
Psmd4-flox
Product ID:
S-CKO-04496
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Psmd4-flox
Strain ID
CKOCMP-19185-Psmd4-B6J-VA
Gene Name
Product ID
S-CKO-04496
Gene Alias
Af1; Mcb1; angiocidin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Psmd4em1flox/Cya mice (Catalog S-CKO-04496) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071664
NCBI RefSeq
NM_001282017
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Psmd4, also known as Rpn10, is a subunit of the 19S proteasome unit and a major ubiquitin receptor of the 26S proteasome. It is involved in feeding target proteins into the catalytic machinery of the 26S proteasome, playing a crucial role in the ubiquitin-dependent proteolytic pathway, which is vital for cellular regulation including cell cycle, differentiation, and apoptosis [2,4,5].
In multiple cancers, Psmd4 has shown significant effects. In hepatocellular carcinoma (HCC), loss of Psmd4 in HCC cells suppressed tumor development in mouse xenograft models. Psmd4 is associated with Akt/GSK-3β/cyclooxygenase2 (COX2) pathway activation, inhibition of p53 promoter activity, and increased p53 degradation, promoting HCC tumor growth [1,5]. In multiple myeloma, inducible knockout or knockdown of Rpn10 (Psmd4) decreased MM cell viability both in vitro and in vivo by triggering polyubiquitinated protein accumulation, cell cycle arrest, and apoptosis [2]. In esophageal cancer, knockdown of Psmd4 induced cell apoptosis, cell cycle arrest, and enhanced endoplasmic reticulum stress response [3]. In epithelial ovarian cancer, down-regulation of Psmd4 increased the sensitivity to carboplatin by inhibiting the NF-κB pathway, attenuating autophagy, and enhancing intracellular ROS accumulation [6]. In chemoresistant colorectal cancer, Psmd4 activated by cytoplasmic localization of Nrf2 may be responsible for chemoresistance via the NF-κB/AKT/β-catenin/ZEB1 cascades [7].
In conclusion, Psmd4 is essential in the ubiquitin-proteasome pathway and significantly impacts various cellular processes. Through gene knockout or knockdown studies in different cancer models, it has been revealed that Psmd4 plays a promoting role in the development and progression of multiple cancers, making it a potential therapeutic target for these diseases.
References:
1. Zhang, Jiamin, Fang, Shu, Rong, Fanghao, Cui, Huixian, Hao, Peipei. 2023. PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition. In Human cell, 36, 1755-1772. doi:10.1007/s13577-023-00935-1. https://pubmed.ncbi.nlm.nih.gov/37336868/
2. Du, Ting, Song, Yan, Ray, Arghya, Chauhan, Dharminder, Anderson, Kenneth C. . Ubiquitin receptor PSMD4/Rpn10 is a novel therapeutic target in multiple myeloma. In Blood, 141, 2599-2614. doi:10.1182/blood.2022017897. https://pubmed.ncbi.nlm.nih.gov/36630605/
3. Ma, Ai-Guo, Yu, Li-Mei, Zhao, Hong, Tian, Xiang-Yu, Wang, Qing. 2019. PSMD4 regulates the malignancy of esophageal cancer cells by suppressing endoplasmic reticulum stress. In The Kaohsiung journal of medical sciences, 35, 591-597. doi:10.1002/kjm2.12093. https://pubmed.ncbi.nlm.nih.gov/31162820/
4. AydoĞan TÜrkoĞlu, Sümeyye, Dayi, Gizem, KÖÇkar, Feray. 2020. Upregulation of PSMD4 gene by hypoxia in prostate cancer cells. In Turkish journal of biology = Turk biyoloji dergisi, 44, 275-283. doi:10.3906/biy-2002-71. https://pubmed.ncbi.nlm.nih.gov/33110365/
5. Cai, Mei-Juan, Cui, Yu, Fang, Min, Pang, Fei, Cui, Xiang-Dan. 2019. Inhibition of PSMD4 blocks the tumorigenesis of hepatocellular carcinoma. In Gene, 702, 66-74. doi:10.1016/j.gene.2019.03.063. https://pubmed.ncbi.nlm.nih.gov/30930224/
6. Li, Ying, Zhou, Qin, Shen, Jing, Zhu, Lixia. . Down-regulation of PSMD4 can attenuate autophagy, enhance the accumulation of intracellular ROS, and increase the sensitivity of epithelial ovarian cancer to carboplatin by inhibiting the NF-κB pathway. In Translational cancer research, 10, 4756-4772. doi:10.21037/tcr-21-1389. https://pubmed.ncbi.nlm.nih.gov/35116329/
7. Cheng, Ya-Min, Lin, Po-Lin, Wu, De-Wei, Huang, Chi-Chou, Lee, Huei. 2018. PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2. In Oncotarget, 9, 26342-26352. doi:10.18632/oncotarget.25254. https://pubmed.ncbi.nlm.nih.gov/29899863/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen