C57BL/6NCya-Ptprcem1flox/Cya
Common Name:
Ptprc-flox
Product ID:
S-CKO-04589
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ptprc-flox
Strain ID
CKOCMP-19264-Ptprc-B6N-VA
Gene Name
Product ID
S-CKO-04589
Gene Alias
B220; CD45R; Cd45; L-CA; Ly-5; Lyt-4; T200; loc
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ptprcem1flox/Cya mice (Catalog S-CKO-04589) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000183301
NCBI RefSeq
XM_006529257
Target Region
Exon 13~14
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Ptprc, also known as CD45, is a transmembrane glycoprotein. It is an essential regulator of T and B cell antigen receptor-mediated activation, playing a key role in maintaining the equilibrium between protein tyrosine kinase and phosphatase activity. Disruptions can lead to immunodeficiency, autoimmunity, or malignancy. It is expressed on almost all haematopoietic cells except mature erythrocytes and functions upstream of a large signalling network [1].
In cutaneous melanoma, PTPRC is positively correlated with positive prognosis, and its knockdown enhances melanoma cell migration, invasion, and proliferation [2]. In breast cancer, PTPRC promotes CD8+ T cell-mediated tumor immunity and drug sensitivity, and intracellular down-regulation of PTPRC enhances paclitaxel tolerance in triple-negative breast cancer cell lines [3]. In pediatric acute myeloid leukemia, high PTPRC expression is associated with a worse overall survival rate and is an independent adverse prognostic factor, and its expression level is correlated with the infiltration level of activated dendritic cells [5]. In rheumatoid arthritis, the PTPRC rs10919563 A allele shows a poor response to anti-TNF therapy [4]. In HIV-1 seroconverters, PTPRC and interferon-response pathways have significantly higher expression prior to infection [6]. In Parkinson's and progressive supranuclear palsy disorders, PTPRC mRNA is downregulated in PSP patients compared to PD and healthy controls, and it may be useful as part of a biosignature to stratify patients according to disease severity and progression [7].
In conclusion, Ptprc is crucial for the regulation of immune cell functions. Studies on Ptprc in various diseases such as melanoma, breast cancer, leukemia, rheumatoid arthritis, HIV-1 infection, and neurodegenerative disorders have provided insights into its role in disease prognosis, immune cell infiltration, and response to therapy. These findings highlight the importance of Ptprc as a potential biomarker and therapeutic target in multiple disease areas.
References:
1. Al Barashdi, Maryam Ahmed, Ali, Ahlam, McMullin, Mary Frances, Mills, Ken. 2021. Protein tyrosine phosphatase receptor type C (PTPRC or CD45). In Journal of clinical pathology, 74, 548-552. doi:10.1136/jclinpath-2020-206927. https://pubmed.ncbi.nlm.nih.gov/34039664/
2. Li, Xuemei, Yue, Zhanghui, Wang, Dan, Zhou, Lu. 2023. PTPRC functions as a prognosis biomarker in the tumor microenvironment of cutaneous melanoma. In Scientific reports, 13, 20617. doi:10.1038/s41598-023-46794-6. https://pubmed.ncbi.nlm.nih.gov/37996489/
3. Li, Pengping, Wang, Wei, Wang, Shaowen, Jin, Haigang, Wang, Zhenyu. 2023. PTPRC promoted CD8+ T cell mediated tumor immunity and drug sensitivity in breast cancer: based on pan-cancer analysis and artificial intelligence modeling of immunogenic cell death-based drug sensitivity stratification. In Frontiers in immunology, 14, 1145481. doi:10.3389/fimmu.2023.1145481. https://pubmed.ncbi.nlm.nih.gov/37388747/
4. Lee, Young Ho, Bae, Sang-Cheol. 2016. Associations between PTPRC rs10919563 A/G and FCGR2A R131H polymorphisms and responsiveness to TNF blockers in rheumatoid arthritis: a meta-analysis. In Rheumatology international, 36, 837-44. doi:10.1007/s00296-016-3476-5. https://pubmed.ncbi.nlm.nih.gov/27074847/
5. Guo, Gaili, Li, Banban, Li, Qianpeng, Li, Chunpu, Guo, Dongmei. . PTPRC Overexpression Predicts Poor Prognosis and Correlates with Immune Cell Infiltration in Pediatric Acute Myeloid Leukemia. In Clinical laboratory, 68, . doi:10.7754/Clin.Lab.2021.210940. https://pubmed.ncbi.nlm.nih.gov/35975530/
6. Li, Yunqi, Lefebvre, Francois, Nakku-Joloba, Edith, Lingappa, Jairam R, Mackelprang, Romel D. . Upregulation of PTPRC and Interferon Response Pathways in HIV-1 Seroconverters Prior to Infection. In The Journal of infectious diseases, 227, 714-719. doi:10.1093/infdis/jiac498. https://pubmed.ncbi.nlm.nih.gov/36637125/
7. Bottero, Virginie, Santiago, Jose A, Potashkin, Judith A. . PTPRC Expression in Blood is Downregulated in Parkinson's and Progressive Supranuclear Palsy Disorders. In Journal of Parkinson's disease, 8, 529-537. doi:10.3233/JPD-181391. https://pubmed.ncbi.nlm.nih.gov/30248063/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen