C57BL/6JCya-Rasgrp1em1flox/Cya
Common Name:
Rasgrp1-flox
Product ID:
S-CKO-04704
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rasgrp1-flox
Strain ID
CKOCMP-19419-Rasgrp1-B6J-VA
Gene Name
Product ID
S-CKO-04704
Gene Alias
Rasgrp; calDAG-GEFII
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rasgrp1em1flox/Cya mice (Catalog S-CKO-04704) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102534
NCBI RefSeq
NM_011246
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Rasgrp1, a Ras guanine nucleotide exchange factor, is an essential regulator of lymphocyte receptor signaling. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor, activating RAS by exchanging bound GDP for GTP, with downstream effects at the cellular level [4]. Genetic models, like mouse models, have been crucial for studying its functions.
In mice, Rasgrp1 deletion leads to defective T lymphocyte development [1]. Rasgrp1 -deficient patients suffer from immune deficiency, and its gene has been linked to autoimmunity. Diminished Rasgrp1 expression in C57BL/6 mice causes defective T lymphocyte selection, and the severity of inflammatory disease inversely correlates with its expression levels [1]. In psoriasis mouse models, Rasgrp1 deficiency and overexpression influence imiquimod-induced psoriasis-like manifestations and skin inflammation, as VEGF mediates psoriatic inflammation through the RasGRP1 -AKT -NF-κB pathway [2]. Also, RasGRP1 promotes the acute inflammatory response by sponging let -7a to increase IL-6 expression at the mRNA level, and restricts inflammation-associated cancer cell growth by disrupting the EGFR -SOS1 -Ras -AKT signaling pathway at the protein level [3].
In conclusion, Rasgrp1 plays a vital role in lymphocyte receptor signaling, T lymphocyte development, and immune-related processes. Mouse models with Rasgrp1 knockout or conditional knockout have revealed its significance in autoimmunity, psoriasis, and the regulation of the acute inflammatory response and inflammation-associated cancer, contributing to our understanding of these disease areas.
References:
1. Baars, Matthijs J D, Douma, Thera, Simeonov, Dimitre R, Roose, Jeroen P, Vercoulen, Yvonne. 2020. Dysregulated RASGRP1 expression through RUNX1 mediated transcription promotes autoimmunity. In European journal of immunology, 51, 471-482. doi:10.1002/eji.201948451. https://pubmed.ncbi.nlm.nih.gov/33065764/
2. Mao, Yiwen, Ge, Huiyao, Chen, Weiwei, Li, Bao, Sun, Liangdan. 2023. RasGRP1 influences imiquimod-induced psoriatic inflammation via T-cell activation in mice. In International immunopharmacology, 122, 110590. doi:10.1016/j.intimp.2023.110590. https://pubmed.ncbi.nlm.nih.gov/37429143/
3. Wang, Cong, Li, Xue, Xue, Binbin, Wang, Jianli, Tang, Songqing. 2022. RasGRP1 promotes the acute inflammatory response and restricts inflammation-associated cancer cell growth. In Nature communications, 13, 7001. doi:10.1038/s41467-022-34659-x. https://pubmed.ncbi.nlm.nih.gov/36385095/
4. Fan, Shangzhi, Kang, Bo, Li, Shaoqian, Chen, Danjun, Zhou, Jiecan. 2024. Exploring the multifaceted role of RASGRP1 in disease: immune, neural, metabolic, and oncogenic perspectives. In Cell cycle (Georgetown, Tex.), 23, 722-746. doi:10.1080/15384101.2024.2366009. https://pubmed.ncbi.nlm.nih.gov/38865342/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen