C57BL/6JCya-Trim27em1flox/Cya
Common Name
Trim27-flox
Product ID
S-CKO-04783
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-19720-Trim27-B6J-VA
When using this mouse strain in a publication, please cite “Trim27-flox Mouse (Catalog S-CKO-04783) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Trim27-flox
Strain ID
CKOCMP-19720-Trim27-B6J-VA
Gene Name
Product ID
S-CKO-04783
Gene Alias
Rfp, Gm19403
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 13
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021761
NCBI RefSeq
NM_009054
Target Region
Exon 5~6
Size of Effective Region
~1.6 kb
Overview of Gene Research
Trim27, a RING-mediated E3 ubiquitin ligase and a member of the TRIM protein family, can mark other proteins like PTEN, IκBα and p53 for degradation, regulating multiple signaling pathways related to cell proliferation, differentiation, and apoptosis [3]. It is involved in pathways such as Wnt/β -catenin, autophagy-related pathways, and endosomal trafficking regulation.
Knockout of Trim27 in mice causes IBS-like symptoms, including increased visceral hyperalgesia and abnormal stool features, revealing its role in maintaining gut homeostasis through promoting intestinal stem cell self-renewal via stabilizing β -catenin and activating Wnt/β -catenin signaling [1]. In tuberculosis, Trim27 enhances host autophagy flux in an E3 ligase activity-independent manner to elicit protective immunity against Mycobacterium tuberculosis [2]. In cancer-related studies, Trim27 knockout in mouse models shows differential regulation of tumorigenesis and metastasis. It cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis [4].
In conclusion, Trim27 plays crucial roles in maintaining gut homeostasis, anti-tuberculosis immunity, and the regulation of autophagy and tumorigenesis. Gene knockout mouse models have been instrumental in revealing these functions, providing potential therapeutic targets for diseases such as IBS, tuberculosis, and cancer.
References：
1. Wang, Jing, Zhao, Dongdong, Lei, Zehui, Zhang, Lingqiang, Liu, Cui Hua. 2023. TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal. In Cellular & molecular immunology, 20, 158-174. doi:10.1038/s41423-022-00963-1. https://pubmed.ncbi.nlm.nih.gov/36596873/
2. Zhao, Dongdong, Qiang, Lihua, Lei, Zehui, Liu, Cui Hua, Wang, Jing. 2024. TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux. In Autophagy, 20, 1483-1504. doi:10.1080/15548627.2024.2321831. https://pubmed.ncbi.nlm.nih.gov/38390831/
3. Yu, Chengpeng, Rao, Dean, Wang, Tiantian, Zhang, Lei, Huang, Wenjie. 2022. Emerging roles of TRIM27 in cancer and other human diseases. In Frontiers in cell and developmental biology, 10, 1004429. doi:10.3389/fcell.2022.1004429. https://pubmed.ncbi.nlm.nih.gov/36200036/
4. Yang, Yi, Zhu, Yifu, Zhou, Shuai, Wu, Mian, Chen, Song. 2022. TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis. In The EMBO journal, 41, e109777. doi:10.15252/embj.2021109777. https://pubmed.ncbi.nlm.nih.gov/35670107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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