C57BL/6NCya-St8sia1em1flox/Cya
Common Name:
St8sia1-flox
Product ID:
S-CKO-05054
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
St8sia1-flox
Strain ID
CKOCMP-20449-St8sia1-B6N-VA
Gene Name
Product ID
S-CKO-05054
Gene Alias
9330109E03Rik; GD3S; Sia-T; Siat8; Siat8a
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-St8sia1em1flox/Cya mice (Catalog S-CKO-05054) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032421
NCBI RefSeq
NM_011374
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
St8sia1, also known as GD3 synthase, is a ganglioside synthesis enzyme. It is involved in the synthesis of GD2, a key molecule in the context of anti-GD2 antibody-based immunotherapies. Its functions are related to multiple signaling pathways, including Wnt/β-catenin, FAK/Akt/mTOR, and JAK/STAT, which are crucial for cell growth, survival, and metastasis [1,2,4].
In neuroblastoma, a forced adrenergic-to-mesenchymal transition leads to down-regulation of St8sia1, reducing GD2 expression and conferring resistance to anti-GD2 antibody. Pharmacologic inhibition of EZH2 can re-express St8sia1, restoring GD2 expression and sensitivity to the antibody [1].
In triple-negative breast cancer, increased St8sia1 levels are associated with chemoresistance, and its inhibition sensitizes the cancer cells to chemotherapy by suppressing Wnt/β-catenin and FAK/Akt/mTOR pathways [2].
In rectal adenocarcinoma, overexpression of St8sia1 inhibits tumor progression via TGF-β1 signaling and promotes the tumoricidal effects of CD8+ T cells [3].
In bladder cancer, St8sia1 overexpression attenuates cell proliferation, migration, and invasion by blocking the JAK/STAT signaling pathway [4].
In TNBC, St8sia1 is highly expressed, positively correlated with BCSC-associated genes, and its knockout blocks in vivo tumor growth and metastasis by activating the FAK-AKT-mTOR signaling pathway [5].
In mice, St8sia1-deficiency in gliomas leads to reduced disease severity, altering the tumor environment [6].
In conclusion, St8sia1 plays diverse roles in multiple cancer types, influencing tumor growth, metastasis, and response to therapies. Studies using gene-knockout models, especially in mice, have been instrumental in revealing these functions, highlighting St8sia1 as a potential therapeutic target in cancer treatment.
References：
1. Mabe, Nathaniel W, Huang, Min, Dalton, Guillermo N, Stegmaier, Kimberly, Majzner, Robbie G. 2022. Transition to a mesenchymal state in neuroblastoma confers resistance to anti-GD2 antibody via reduced expression of ST8SIA1. In Nature cancer, 3, 976-993. doi:10.1038/s43018-022-00405-x. https://pubmed.ncbi.nlm.nih.gov/35817829/
2. Wan, H, Li, Z, Wang, H, Cai, F, Wang, L. 2020. ST8SIA1 inhibition sensitizes triple negative breast cancer to chemotherapy via suppressing Wnt/β-catenin and FAK/Akt/mTOR. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 23, 902-910. doi:10.1007/s12094-020-02484-7. https://pubmed.ncbi.nlm.nih.gov/32939659/
3. Zhang, Chang, Wang, Yeli, Yu, Yao, Xiao, Xiao, Hao, Leilei. 2024. Overexpression of ST8Sia1 inhibits tumor progression by TGF-β1 signaling in rectal adenocarcinoma and promotes the tumoricidal effects of CD8+ T cells by granzyme B and perforin. In Annals of medicine, 57, 2439539. doi:10.1080/07853890.2024.2439539. https://pubmed.ncbi.nlm.nih.gov/39656552/
4. Yu, Shengjin, Wang, Shidan, Sun, Xiaoxin, Yang, Deyong, Jiang, Yu. 2021. ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway. In Oncology letters, 22, 736. doi:10.3892/ol.2021.12997. https://pubmed.ncbi.nlm.nih.gov/34429775/
5. Nguyen, Khoa, Yan, Yuanqing, Yuan, Bin, Andreeff, Michael, Battula, V Lokesh. 2018. ST8SIA1 Regulates Tumor Growth and Metastasis in TNBC by Activating the FAK-AKT-mTOR Signaling Pathway. In Molecular cancer therapeutics, 17, 2689-2701. doi:10.1158/1535-7163.MCT-18-0399. https://pubmed.ncbi.nlm.nih.gov/30237308/
6. Zhang, Pu, Ohkawa, Yuki, Yamamoto, Satoko, Furukawa, Keiko, Furukawa, Koichi. . St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity. In Nagoya journal of medical science, 83, 535-549. doi:10.18999/nagjms.83.3.535. https://pubmed.ncbi.nlm.nih.gov/34552288/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen