C57BL/6JCya-Snai1em1flox/Cya
Common Name
Snai1-flox
Product ID
S-CKO-05146
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-20613-Snai1-B6J-VA
When using this mouse strain in a publication, please cite “Snai1-flox Mouse (Catalog S-CKO-05146) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Snai1-flox
Strain ID
CKOCMP-20613-Snai1-B6J-VA
Gene Name
Product ID
S-CKO-05146
Gene Alias
Sna, Sna1, Snail, Snail1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000052631
NCBI RefSeq
NM_011427
Target Region
Exon 1~2
Size of Effective Region
~1.8 kb
Overview of Gene Research
Snai1, a zinc finger transcription factor, is a master regulator of epithelial-mesenchymal transition (EMT) [2]. It also drives various cancer-related processes such as cell invasion, survival, immune regulation, stem cell properties, and metabolic regulation [2]. Snai1's regulation occurs at transcriptional, translational, and predominantly post-translational levels like phosphorylation, acetylation, and ubiquitination [2].
In liver-related studies, hepatocyte-specific deletion of both Snai1 and Snai2 (but not one alone) suppresses liver cyclin A2/D1 expression and regenerative hepatocyte proliferation after hepatectomy or CCl4 treatments, while augmenting CCl4-stimulated HSC activation and liver fibrosis. This indicates that Snai1, along with Snai2, promotes liver regeneration and suppresses liver fibrosis, likely via histone modifications [1]. In breast cancer stem cells, stable overexpression of Snai1 in MCF-7 cells induced the expression of EMT-related genes, increased migration, invasion, and in vitro tumorigenesis. SnaI1 positively regulated genes like ALCAM, MMP2, MMP13, MMP14, VCAN, ANKRD1, KRT16, CTGF, TGFRIIβ, PROCR, and negatively regulated CDH1, DSP, and DSC3B, leading to EMT [3].
In summary, Snai1 is crucial in EMT and various cancer-related processes. Its functions in liver regeneration and fibrosis, as well as in breast cancer stem cell-related EMT, are revealed through gene-knockout-based studies. These findings help in understanding the mechanisms of diseases like liver fibrosis and cancer, providing potential targets for therapeutic intervention.
References：
1. Wang, Pingping, Kang, Qianqian, Wu, Wen-Shu, Rui, Liangyou. 2024. Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice. In Cell reports, 43, 113875. doi:10.1016/j.celrep.2024.113875. https://pubmed.ncbi.nlm.nih.gov/38451818/
2. Dong, Bo, Wu, Yadi. 2021. Epigenetic Regulation and Post-Translational Modifications of SNAI1 in Cancer Metastasis. In International journal of molecular sciences, 22, . doi:10.3390/ijms222011062. https://pubmed.ncbi.nlm.nih.gov/34681726/
3. Singh, Digvijay, Deshmukh, Rohit K, Das, Amitava. 2021. SNAI1-mediated transcriptional regulation of epithelial-to-mesenchymal transition genes in breast cancer stem cells. In Cellular signalling, 87, 110151. doi:10.1016/j.cellsig.2021.110151. https://pubmed.ncbi.nlm.nih.gov/34537302/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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