C57BL/6JCya-Snrkem1flox/Cya
Common Name:
Snrk-flox
Product ID:
S-CKO-05155
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Snrk-flox
Strain ID
CKOCMP-20623-Snrk-B6J-VA
Gene Name
Product ID
S-CKO-05155
Gene Alias
2010012F07Rik; E030034B15; mKIAA0096
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snrkem1flox/Cya mice (Catalog S-CKO-05155) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000118886
NCBI RefSeq
NM_133741
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
SnRK, short for sucrose non-fermenting 1-related kinase, is a serine/threonine kinase and a member of the AMP-activated protein kinase (AMPK) family. It is involved in metabolic regulatory mechanisms, playing a crucial role in maintaining cellular metabolic homeostasis. SnRK participates in multiple signaling pathways, such as those related to metabolism, DNA damage response (DDR), and autophagy, and is important for various biological processes including plant growth, development, stress responses, and mammalian cardiovascular function and lipid homeostasis [1,2,3,4]. Genetic models like knockout (KO) and conditional knockout (CKO) mice are valuable tools for studying its functions.
In cardiac-specific Snrk-/-mice, transaortic banding leads to worse cardiac function, increased cardiac hypertrophy, and elevated DDR marker pH2AX, indicating SnRK's role in cardiac hypertrophy and DNA damage [1]. In MAFLD, SnRK-deficient mice show fatty acid oxidation damage and persistent liver lipid accumulation, and pharmacological inhibition of the mTOR pathway in these mice restores autophagy and improves lipid accumulation, suggesting SnRK's importance in liver lipid homeostasis [3]. Conditional knockout of Snrk in mouse cardiomyocytes causes atrial fibrosis and heart failure, with Snrk knockdown cells showing more TGFβ1 secretion, demonstrating its role in regulating cardiac fibrosis [5]. In Snrk global heterozygous knockout and endothelial cell-specific Snrk deletion mice, retina angiogenesis and neovessel formation after hindlimb ischemia are suppressed, revealing SnRK's role in angiogenesis [6]. Also, cardiomyocyte-specific Snrk knockout in adult mice leads to heart failure, increased inflammation, and fibrosis, highlighting SnRK as a cardiomyocyte-specific repressor of cardiac inflammation and fibrosis [7].
In conclusion, SnRK is a key regulator in multiple biological processes. Studies using SnRK KO/CKO mouse models have revealed its significance in diseases like cardiac hypertrophy, MAFLD, cardiac fibrosis, and angiogenesis, as well as its role in maintaining cardiac function by suppressing inflammation. These findings provide insights into potential therapeutic strategies targeting SnRK for related diseases.
References:
1. Stanczyk, Paulina J, Tatekoshi, Yuki, Shapiro, Jason S, Chang, Hsiang-Chun, Ardehali, Hossein. 2023. DNA Damage and Nuclear Morphological Changes in Cardiac Hypertrophy Are Mediated by SNRK Through Actin Depolymerization. In Circulation, 148, 1582-1592. doi:10.1161/CIRCULATIONAHA.123.066002. https://pubmed.ncbi.nlm.nih.gov/37721051/
2. Son, Seungmin, Park, Sang Ryeol. 2023. The rice SnRK family: biological roles and cell signaling modules. In Frontiers in plant science, 14, 1285485. doi:10.3389/fpls.2023.1285485. https://pubmed.ncbi.nlm.nih.gov/38023908/
3. Lin, Shan, Qiu, Xiusheng, Fu, Xiaoying, Guan, Haixia, Lai, Shuiqing. 2024. SNRK modulates mTOR-autophagy pathway for liver lipid homeostasis in MAFLD. In Molecular therapy : the journal of the American Society of Gene Therapy, 33, 279-296. doi:10.1016/j.ymthe.2024.11.016. https://pubmed.ncbi.nlm.nih.gov/39521960/
4. Thirugnanam, Karthikeyan, Ramchandran, Ramani. 2020. SNRK: a metabolic regulator with multifaceted role in development and disease. In Vessel plus, 4, . doi:. https://pubmed.ncbi.nlm.nih.gov/32968716/
5. Thirugnanam, Karthikeyan, Rizvi, Farhan, Jahangir, Arshad, Sekine, Hidekazu, Ramchandran, Ramani. 2024. SNRK regulates TGFβ levels in atria to control cardiac fibrosis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.09.24.612951. https://pubmed.ncbi.nlm.nih.gov/39386731/
6. Lu, Qiulun, Xie, Zhonglin, Yan, Chenghui, Ramchandran, Ramani, Zou, Ming-Hui. 2017. SNRK (Sucrose Nonfermenting 1-Related Kinase) Promotes Angiogenesis In Vivo. In Arteriosclerosis, thrombosis, and vascular biology, 38, 373-385. doi:10.1161/ATVBAHA.117.309834. https://pubmed.ncbi.nlm.nih.gov/29242271/
7. Thirugnanam, Karthikeyan, Cossette, Stephanie M, Lu, Qiulun, Zou, Ming-Hui, Ramchandran, Ramani. 2019. Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart. In Journal of the American Heart Association, 8, e012792. doi:10.1161/JAHA.119.012792. https://pubmed.ncbi.nlm.nih.gov/31718444/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen