C57BL/6JCya-Sox9em1flox/Cya
Common Name:
Sox9-flox
Product ID:
S-CKO-05191
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Sox9-flox
Strain ID
CKOCMP-20682-Sox9-B6J-VA
Gene Name
Product ID
S-CKO-05191
Gene Alias
2010306G03Rik; mKIAA4243; mSox9
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sox9em1flox/Cya mice (Catalog S-CKO-05191) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000579
NCBI RefSeq
NM_011448
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Sox9, a transcription factor, is essential for the development of multiple organs such as bone, testis, heart, lung, pancreas, intestine, and the nervous system [1]. It is involved in various molecular genetic pathways crucial for organ development, maintenance, and function. Mutations in the human SOX9 gene lead to campomelic dysplasia, often accompanied by 46, XY sex reversal [1]. Genetic models, like KO/CKO mouse models, have been instrumental in studying its functions.
In mouse gonadogenesis, Sox9 is detected in the male gonad at 11.5 days postcoitus. Germ-line knockout of Sox9 using a conditional null allele in mice shows that Sox9-/-mice die soon after 11.5 dpc due to cardiovascular defects. In vitro culture of XY Sox9-/-urogenital ridges results in gonads lacking testicular cords and Sertoli cell marker expression but with ovarian-specific marker expression, indicating Sox9 is essential for testis formation [2]. In pancreatic development, transgenic mouse models have dissected the role of Sox9, which is considered a master regulator. It maintains pancreatic progenitors, initiates endocrine differentiation, and maintains pancreatic ductal identity. It has also been linked to pancreatic cancer initiation [3].
In conclusion, Sox9 plays diverse and essential roles in organ development, including testis determination and pancreatic development. Studies using Sox9 KO/CKO mouse models have provided valuable insights into its functions in these processes and their associated diseases, such as campomelic dysplasia and pancreatic cancer.
References:
1. Ming, Zhenhua, Vining, Brittany, Bagheri-Fam, Stefan, Harley, Vincent. 2022. SOX9 in organogenesis: shared and unique transcriptional functions. In Cellular and molecular life sciences : CMLS, 79, 522. doi:10.1007/s00018-022-04543-4. https://pubmed.ncbi.nlm.nih.gov/36114905/
2. Kobayashi, Akio, Chang, Hao, Chaboissier, Marie-Christine, Schedl, Andreas, Behringer, Richard R. . Sox9 in testis determination. In Annals of the New York Academy of Sciences, 1061, 9-17. doi:. https://pubmed.ncbi.nlm.nih.gov/16467253/
3. Seymour, Philip A. 2014. Sox9: a master regulator of the pancreatic program. In The review of diabetic studies : RDS, 11, 51-83. doi:10.1900/RDS.2014.11.51. https://pubmed.ncbi.nlm.nih.gov/25148367/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen