C57BL/6JCya-Tfe3em1flox/Cya
Common Name
Tfe3-flox
Product ID
S-CKO-05454
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-209446-Tfe3-B6J-VA
When using this mouse strain in a publication, please cite “Tfe3-flox Mouse (Catalog S-CKO-05454) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tfe3-flox
Strain ID
CKOCMP-209446-Tfe3-B6J-VA
Gene Name
Product ID
S-CKO-05454
Gene Alias
Tcfe3, Tfe-3, mTFE3, bHLHe33, F830016E06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000077680
NCBI RefSeq
NM_172472
Target Region
Exon 4~6
Size of Effective Region
~2.7 kb
Overview of Gene Research
TFE3, also known as transcription factor binding to IGHM enhancer 3, is a member of the MiT family of the bHLH-leucine zipper transcription factor. It plays a crucial role in various biological processes such as autophagy, lysosomal activity, and metabolic regulation [2,5]. It participates in energy metabolism by regulating glucose and lipid metabolism, mitochondrial metabolism, and autophagy pathways [5]. TFE3 also has a significant impact on processes like insulin gene expression and glucose homeostasis [6].
In renal cell carcinoma, TFE3-translocation renal cell carcinoma (TFE3-tRCC) is a rare subtype. It is highly heterogeneous, both clinicopathologically and genotypically. ASPSCR1-TFE3 fusion and certain somatic copy number alterations are related to aggressive features and poor outcomes [1]. TFE3-immunopositive papillary renal cell carcinoma without TFE3 gene rearrangement shows a poorer prognosis and is associated with autophagy/lysosome protein expressions [3]. In TFE3-rearranged perivascular epithelioid cell tumors (PEComas), about 11% cases are diagnosed post-chemotherapy, and most have a poor prognosis [4]. Also, in Birt-Hogg-Dubé (BHD) syndrome, TFE3, along with TFEB, drives kidney cystogenesis and tumorigenesis [7].
In conclusion, TFE3 is a key regulator in multiple biological processes. Its role in autophagy, metabolic regulation, and especially in various kidney-related diseases and tumors has been well-demonstrated through studies. The research on TFE3-related models has provided valuable insights into understanding the mechanisms of these diseases and may guide the development of new therapeutic strategies.
References：
1. Sun, Guangxi, Chen, Junru, Liang, Jiayu, Chen, Ni, Zeng, Hao. 2021. Integrated exome and RNA sequencing of TFE3-translocation renal cell carcinoma. In Nature communications, 12, 5262. doi:10.1038/s41467-021-25618-z. https://pubmed.ncbi.nlm.nih.gov/34489456/
2. Paquette, Mathieu, El-Houjeiri, Leeanna, C Zirden, Linda, Siegel, Peter M, Pause, Arnim. 2021. AMPK-dependent phosphorylation is required for transcriptional activation of TFEB and TFE3. In Autophagy, 17, 3957-3975. doi:10.1080/15548627.2021.1898748. https://pubmed.ncbi.nlm.nih.gov/33734022/
3. Takamatsu, Dai, Kohashi, Kenichi, Kiyozawa, Daisuke, Eto, Masatoshi, Oda, Yoshinao. 2023. TFE3-immunopositive papillary renal cell carcinoma: A clinicopathological, immunohistochemical, and genetic study. In Pathology, research and practice, 242, 154313. doi:10.1016/j.prp.2023.154313. https://pubmed.ncbi.nlm.nih.gov/36669395/
4. Argani, Pedram, Gross, John M, Baraban, Ezra, Suurmeijer, Albert J H, Antonescu, Cristina R. 2024. TFE3 -Rearranged PEComa/PEComa-like Neoplasms : Report of 25 New Cases Expanding the Clinicopathologic Spectrum and Highlighting its Association With Prior Exposure to Chemotherapy. In The American journal of surgical pathology, 48, 777-789. doi:10.1097/PAS.0000000000002218. https://pubmed.ncbi.nlm.nih.gov/38597260/
5. Li, Xingyu, Chen, Yongming, Gong, Siqiao, Li, Xiaoyu, Hao, Junfeng. 2023. Emerging roles of TFE3 in metabolic regulation. In Cell death discovery, 9, 93. doi:10.1038/s41420-023-01395-0. https://pubmed.ncbi.nlm.nih.gov/36906611/
6. Pasquier, Adrien, Pastore, Nunzia, D'Orsi, Luca, Napolitano, Gennaro, Ballabio, Andrea. 2023. TFEB and TFE3 control glucose homeostasis by regulating insulin gene expression. In The EMBO journal, 42, e113928. doi:10.15252/embj.2023113928. https://pubmed.ncbi.nlm.nih.gov/37712288/
7. Di Malta, Chiara, Zampelli, Angela, Granieri, Letizia, Linehan, W Marston, Ballabio, Andrea. 2023. TFEB and TFE3 drive kidney cystogenesis and tumorigenesis. In EMBO molecular medicine, 15, e16877. doi:10.15252/emmm.202216877. https://pubmed.ncbi.nlm.nih.gov/36987696/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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