C57BL/6JCya-Kcnj14em1flox/Cya
Common Name:
Kcnj14-flox
Product ID:
S-CKO-05580
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Kcnj14-flox
Strain ID
CKOCMP-211480-Kcnj14-B6J-VA
Gene Name
Product ID
S-CKO-05580
Gene Alias
A930026G01Rik; IRK4; Kir2.4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kcnj14em1flox/Cya mice (Catalog S-CKO-05580) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071937
NCBI RefSeq
NM_145963
Target Region
Exon 2~3
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
Kcnj14, also known as Kir2.4, encodes an inwardly rectifying potassium channel. This channel is involved in regulating membrane potential, and is associated with multiple biological processes. For example, in colonic muscles, it contributes to the regulation of resting potentials and excitability through a Ba²⁺ -sensitive, inwardly rectifying K⁺ conductance, likely formed by heterotetramers of Kir2 gene products [3].
In cancer research, knockdown of Kcnj14 significantly inhibited the proliferation and migration of colorectal cells. Kcnj14 expression was increased in colorectal cancer (CRC) tissues and cell lines, reducing patients' overall survival time. It was also found to be dysregulated in various cancers, and was linked to RNA and DNA stemness, immunological checkpoints, suppressor cells, tumour mutation burden, microsatellite instability, neoantigen, and programmed death ligand 1. In CRC, its expression was related to immune cell infiltration and the mTOR signalling pathway [1,2].
In conclusion, Kcnj14 plays a significant role in maintaining the normal physiological function of colonic muscles by regulating membrane potential. In addition, its abnormal expression is closely related to the development and progression of cancers, especially CRC. The study of Kcnj14 knockdown in CRC cells provides valuable insights into its role in cancer, potentially offering new targets for cancer treatment.
References:
1. Li, Bin, Ge, Ning, Pan, Zhongping, Li, Mengyi, Luo, Shuping. 2022. KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells. In BMC medical genomics, 15, 194. doi:10.1186/s12920-022-01351-4. https://pubmed.ncbi.nlm.nih.gov/36100894/
2. Alasiri, Glowi. 2023. Comprehensive Analysis of KCNJ14 Potassium Channel as a Biomarker for Cancer Progression and Development. In International journal of molecular sciences, 24, . doi:10.3390/ijms24032049. https://pubmed.ncbi.nlm.nih.gov/36768373/
3. Huang, Xu, Lee, Si Hyung, Lu, Hongli, Sanders, Kenton M, Koh, Sang Don. 2017. Molecular and functional characterization of inwardly rectifying K+ currents in murine proximal colon. In The Journal of physiology, 596, 379-391. doi:10.1113/JP275234. https://pubmed.ncbi.nlm.nih.gov/29205356/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen