C57BL/6JCya-Asap2em1flox/Cya
Common Name:
Asap2-flox
Product ID:
S-CKO-05601
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Asap2-flox
Strain ID
CKOCMP-211914-Asap2-B6J-VA
Gene Name
Product ID
S-CKO-05601
Gene Alias
6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3; PAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asap2em1flox/Cya mice (Catalog S-CKO-05601) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064595
NCBI RefSeq
NM_001135192
Target Region
Exon 6
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
ASAP2, also known as ArfGAP with SH3 Domain, Ankyrin Repeat And PH Domain 2, is a gene involved in regulating vesicular transport, cellular migration, and autophagy [4]. It can activate GTPases and promote receptor tyrosine kinase signaling, and is associated with pathways like HGF/c-MET signaling, which is crucial in hepatocellular carcinoma (HCC) progression [1].
Knockdown of ASAP2 in HCC cells significantly impaired cell proliferation, migration, and invasion, but promoted apoptosis, while overexpression had the opposite effects. In vivo, ASAP2 promoted HCC cell growth and lung metastasis [1]. In pancreatic cancer, ASAP2-knockout cells generated with CRISPR-Cas9 technology showed that ASAP2 promoted tumor growth by facilitating cell cycle progression through phosphorylation of epidermal growth factor receptor (EGFR) [3]. In diabetic nephropathy, inhibition of circASAP2 aggravated the disease, promoting inflammation, oxidative stress, and ferroptosis [2].
In conclusion, ASAP2 plays essential roles in various biological processes and disease conditions. Model-based research, especially gene-knockout studies, has revealed its significance in cancer progression, such as in HCC and pancreatic cancer, and in diabetic nephropathy. Understanding ASAP2 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Ma, Xiao-Lu, Nie, Yan-Yan, Xie, Su-Hong, Guo, Lin, Lu, Ren-Quan. 2023. ASAP2 interrupts c-MET-CIN85 interaction to sustain HGF/c-MET-induced malignant potentials in hepatocellular carcinoma. In Experimental hematology & oncology, 12, 38. doi:10.1186/s40164-023-00393-3. https://pubmed.ncbi.nlm.nih.gov/37061723/
2. Li, Qin, Meng, Xiangjian, Hua, Qiang. 2022. Circ ASAP2 decreased inflammation and ferroptosis in diabetic nephropathy through SOX2/SLC7A11 by miR-770-5p. In Acta diabetologica, 60, 29-42. doi:10.1007/s00592-022-01961-5. https://pubmed.ncbi.nlm.nih.gov/36153434/
3. Fujii, Atsushi, Masuda, Takaaki, Iwata, Michio, Nakamura, Masafumi, Mimori, Koshi. 2021. The novel driver gene ASAP2 is a potential druggable target in pancreatic cancer. In Cancer science, 112, 1655-1668. doi:10.1111/cas.14858. https://pubmed.ncbi.nlm.nih.gov/33605496/
4. Seuter, Sabine, Ryynänen, Jussi, Carlberg, Carsten. 2013. The ASAP2 gene is a primary target of 1,25-dihydroxyvitamin D3 in human monocytes and macrophages. In The Journal of steroid biochemistry and molecular biology, 144 Pt A, 12-8. doi:10.1016/j.jsbmb.2013.08.014. https://pubmed.ncbi.nlm.nih.gov/23999061/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen