C57BL/6JCya-Satb2em1flox/Cya
Common Name
Satb2-flox
Product ID
S-CKO-05656
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-212712-Satb2-B6J-VA
When using this mouse strain in a publication, please cite “Satb2-flox Mouse (Catalog S-CKO-05656) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Satb2-flox
Strain ID
CKOCMP-212712-Satb2-B6J-VA
Gene Name
Product ID
S-CKO-05656
Gene Alias
BAP002, mKIAA1034
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000114415
NCBI RefSeq
NM_139146
Target Region
Exon 7
Size of Effective Region
~1.2 kb
Overview of Gene Research
SATB2, short for special AT-rich binding protein-2, is a member of the matrix attachment region-binding transcription factor family. It plays crucial roles in multiple biological processes such as craniofacial, neural, and osteoblastic differentiation [1,5]. It modulates chromatin architecture to regulate gene expression, and is involved in pathways related to cell pluripotency, self-renewal, and epithelial-mesenchymal transition (EMT) [3].
In mouse models, Satb2 loss in adult mice leads to the conversion of colonic stem cells into small intestine ileal-like stem cells, replacing the colonic mucosa with an ileum-like one. Conversely, SATB2 can confer colonic properties on the mouse ileum. This shows that SATB2 singularly preserves LGR5+ adult colonic stem cell and epithelial identity in mice and humans [2].
In conclusion, SATB2 is a versatile regulator with essential functions in development, especially in maintaining colonic stem cell identity. Mouse models, especially those with Satb2 knockout or conditional knockout, have been instrumental in revealing its role in intestinal tissue identity and may provide insights into diseases related to abnormal intestinal development or cancer where SATB2 is deregulated [2,3,4].
References：
1. Berg, Kyra B, Schaeffer, David F. . SATB2 as an Immunohistochemical Marker for Colorectal Adenocarcinoma: A Concise Review of Benefits and Pitfalls. In Archives of pathology & laboratory medicine, 141, 1428-1433. doi:10.5858/arpa.2016-0243-RS. https://pubmed.ncbi.nlm.nih.gov/28968158/
2. Gu, Wei, Wang, Hua, Huang, Xiaofeng, Shivdasani, Ramesh A, Zhou, Qiao. 2021. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. In Cell stem cell, 29, 101-115.e10. doi:10.1016/j.stem.2021.09.004. https://pubmed.ncbi.nlm.nih.gov/34582804/
3. Roy, Sanjit K, Shrivastava, Anju, Srivastav, Sudesh, Shankar, Sharmila, Srivastava, Rakesh K. 2020. SATB2 is a novel biomarker and therapeutic target for cancer. In Journal of cellular and molecular medicine, 24, 11064-11069. doi:10.1111/jcmm.15755. https://pubmed.ncbi.nlm.nih.gov/32885593/
4. Chen, Qiao Yi, Des Marais, Thomas, Costa, Max. . Deregulation of SATB2 in carcinogenesis with emphasis on miRNA-mediated control. In Carcinogenesis, 40, 393-402. doi:10.1093/carcin/bgz020. https://pubmed.ncbi.nlm.nih.gov/30916759/
5. Huang, Xia, Chen, Qiuman, Luo, Wenping, El Dafrawy, Mostafa, Zhang, Hongmei. 2020. SATB2: A versatile transcriptional regulator of craniofacial and skeleton development, neurogenesis and tumorigenesis, and its applications in regenerative medicine. In Genes & diseases, 9, 95-107. doi:10.1016/j.gendis.2020.10.003. https://pubmed.ncbi.nlm.nih.gov/35005110/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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