C57BL/6JCya-Mlxem1flox/Cya
Common Name
Mlx-flox
Product ID
S-CKO-05826
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-21428-Mlx-B6J-VA
Status
When using this mouse strain in a publication, please cite “Mlx-flox Mouse (Catalog S-CKO-05826) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mlx-flox
Strain ID
CKOCMP-21428-Mlx-B6J-VA
Gene Name
Product ID
S-CKO-05826
Gene Alias
Tf4, Tcfl4, bHLHd13
Background
C57BL/6JCya
Gene Full Name
MAX-like protein X
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000017945
NCBI Transcript ID
NM_011550
Target Region
Exon 3~7
Size of Effective Region
~2.1 kb
Overview of Gene Research
Mlx, also known as Max-like protein X, is a key component in a transcriptional network parallel to the Myc/Max/Mad network. It functions as a heterodimer binding partner for glucose-sensing transcription factors like ChREBP and MondoA. Mlx is involved in multiple metabolic pathways, including lipid and glucose metabolism, and plays a crucial role in maintaining redox balance, and nutrient-sensing mechanisms [2,3,4,5,6]. Genetic models, such as knockout mouse models, are valuable for studying its functions.
In osteosarcoma, knockdown of MLX impairs tumor growth and metastasis, and disrupts redox balance by affecting SLC7A11-mediated cysteine uptake, leading to ferroptosis [1]. In hepatocellular carcinoma, liver-specific knockout of Mlx decreases lipogenic gene expression, lipid levels, and blocks tumorigenesis in multiple models, highlighting its role in the glucose-induced lipogenic program promoting HCC development [3]. In primary human hepatocytes, MLX knockdown alters lipid and glucose metabolism, favoring catabolism over anabolism [2].
In conclusion, Mlx is essential for regulating lipid and glucose metabolism, redox balance, and plays a significant role in diseases like osteosarcoma and hepatocellular carcinoma. The use of KO/CKO mouse models has been instrumental in revealing these functions, providing insights into potential therapeutic targets for these diseases.
References：
1. Guo, Weitang, Wang, Xin, Lu, Bing, Zhao, Wei, Zou, Changye. 2023. Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma. In Cell death & disease, 14, 439. doi:10.1038/s41419-023-05966-y. https://pubmed.ncbi.nlm.nih.gov/37460542/
2. Nagarajan, Shilpa R, Livingstone, Eilidh J, Monfeuga, Thomas, Ruby, Maxwell A, Hodson, Leanne. 2023. MLX plays a key role in lipid and glucose metabolism in humans: Evidence from in vitro and in vivo studies. In Metabolism: clinical and experimental, 144, 155563. doi:10.1016/j.metabol.2023.155563. https://pubmed.ncbi.nlm.nih.gov/37088121/
3. Yu, Aijuan, Yu, Pengcheng, Zhu, Yuwen, Ye, Dan, Yu, Fa-Xing. 2023. Glucose-induced and ChREBP: MLX-mediated lipogenic program promotes hepatocellular carcinoma development. In Oncogene, 42, 3182-3193. doi:10.1038/s41388-023-02831-2. https://pubmed.ncbi.nlm.nih.gov/37684408/
4. Billin, A N, Ayer, D E. . The Mlx network: evidence for a parallel Max-like transcriptional network that regulates energy metabolism. In Current topics in microbiology and immunology, 302, 255-78. doi:. https://pubmed.ncbi.nlm.nih.gov/16620032/
5. Havula, Essi, Hietakangas, Ville. 2017. Sugar sensing by ChREBP/Mondo-Mlx-new insight into downstream regulatory networks and integration of nutrient-derived signals. In Current opinion in cell biology, 51, 89-96. doi:10.1016/j.ceb.2017.12.007. https://pubmed.ncbi.nlm.nih.gov/29278834/
6. Havula, Essi, Hietakangas, Ville. 2012. Glucose sensing by ChREBP/MondoA-Mlx transcription factors. In Seminars in cell & developmental biology, 23, 640-7. doi:10.1016/j.semcdb.2012.02.007. https://pubmed.ncbi.nlm.nih.gov/22406740/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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