C57BL/6JCya-Prdx2em1flox/Cya
Common Name:
Prdx2-flox
Product ID:
S-CKO-06022
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prdx2-flox
Strain ID
CKOCMP-21672-Prdx2-B6J-VA
Gene Name
Product ID
S-CKO-06022
Gene Alias
Band-8; NkefB; PRP; PrxII; TDX1; TPx; TPx-B; TR; TSA; Tdpx1; Torin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prdx2em1flox/Cya mice (Catalog S-CKO-06022) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005292
NCBI RefSeq
NM_011563
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Prdx2, a characteristic 2-Cys enzyme, is one of the most effective scavenger proteins against reactive oxygen species (ROS) and hydrogen peroxide (H₂O₂), protecting cells from oxidative stress. It is involved in multiple signaling pathways and is crucial for maintaining cellular redox balance. Dysregulation of Prdx2 can lead to increased ROS levels and oxidative stress, which are implicated in various diseases [1].
In non-alcoholic steatohepatitis (NASH) research, liver-specific Prdx2 knockout (Prdx2 LKO) female mice fed a methionine-choline deficient diet (MCD) showed a significant increase in hepatic lipid accumulation, inflammation, circulating levels of aspartate aminotransferase (AST), and pro-inflammatory signaling pathways within the liver. There was also an increase in lipid peroxidation, suggesting a protective role of Prdx2 in NASH [2]. In colorectal cancer cells, removal of Prdx2 using short-hairpin RNA vector inhibited cell-cycle progression and autophagy, and Prdx2 promoted cell-cycle progression via activation of the p38 MAPK pathway [3].
In conclusion, Prdx2 is essential for maintaining cellular redox balance and is involved in multiple biological processes. The use of Prdx2 knockout mouse models has revealed its protective role in NASH and its promoting role in colorectal cancer cell-cycle progression. Understanding Prdx2's functions provides insights into the mechanisms of related diseases and may offer potential therapeutic targets.
References:
1. Balasubramanian, Priyanka, Vijayarangam, Varshini, Deviparasakthi, Mangayer Karasi Gopalakrishnan, Wahab, Mugip Rahaman Abdul, Surendran, Hemapreethi. 2024. Implications and progression of peroxiredoxin 2 (PRDX2) in various human diseases. In Pathology, research and practice, 254, 155080. doi:10.1016/j.prp.2023.155080. https://pubmed.ncbi.nlm.nih.gov/38219498/
2. Zhang, Mengqi, Shi, Xiaofeng, Tang, Minglei, Luo, Cheng, Xie, Xiangyang. 2024. PRDX2 deficiency increases MCD-induced nonalcoholic steatohepatitis in female mice. In Biochemical and biophysical research communications, 701, 149589. doi:10.1016/j.bbrc.2024.149589. https://pubmed.ncbi.nlm.nih.gov/38309152/
3. Zheng, Xiangru, Wei, Jinlai, Li, Wenjun, Guo, Jinbao, Fu, Zhongxue. 2020. PRDX2 removal inhibits the cell cycle and autophagy in colorectal cancer cells. In Aging, 12, 16390-16409. doi:10.18632/aging.103690. https://pubmed.ncbi.nlm.nih.gov/32692719/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen