C57BL/6NCya-Alyrefem1flox/Cya
Common Name:
Alyref-flox
Product ID:
S-CKO-06045
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Alyref-flox
Strain ID
CKOCMP-21681-Alyref-B6N-VA
Gene Name
Product ID
S-CKO-06045
Gene Alias
Aly; Ref1; Ref1-I; Refbp1; Tho4; Thoc4
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Alyrefem1flox/Cya mice (Catalog S-CKO-06045) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026125
NCBI RefSeq
NM_011568
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
ALYREF, also known as Aly/REF export factor, is an RNA-binding protein. It plays key roles in mRNA export, pre-mRNA processing and mRNA stability regulation. It is associated with the 5-methylcytosine (m5C) modification pathway in mRNA metabolism, which is crucial for various biological processes [2,3].
In cancer research, ALYREF has been found to have significant impacts. In bladder cancer, ALYREF stabilizes PKM2 mRNA by binding to its m5C sites in 3'-untranslated regions, promoting cell proliferation via PKM2-mediated glycolysis. High ALYREF expression predicts poor survival in bladder cancer patients, and hypoxia-inducible factor-1alpha (HIF-1α) can up-regulate PKM2 expression indirectly through ALYREF activation [1].
In urothelial bladder cancer, ALYREF enhances cell proliferation and invasion in an m5C-dependent manner, by specifically binding to hypermethylated m5C sites in RABL6 and TK1 mRNA, promoting their splicing and stabilization. High co-expression of ALYREF/RABL6/TK1 axis is associated with poor overall survival [5].
In non-small cell lung cancer, LINC02159 binds to ALYREF to enhance the stability of YAP1 mRNA via m5C modification, promoting cancer progression [4].
In colorectal cancer, ALYREF forms a complex with ELAVL1 to promote m5C recognition and nuclear export of RPS6KB2 and RPTOR mRNAs, supporting cancer growth and migration, and high ALYREF protein expression predicts poor prognosis [6].
In nasopharyngeal carcinoma, ALYREF up-regulates NOTCH1 expression by enhancing its RNA stability in an m5C-modification-dependent manner, promoting cancer metastasis [7].
In breast cancer, high ALYREF expression is associated with poor prognosis, and ALYREF promotes cancer cell viability and decreases apoptosis by regulating EZH2 [8].
In conclusion, ALYREF is essential for mRNA-related regulatory processes. Through gene-knockout or conditional-knockout mouse models and in vivo studies in cancer research, ALYREF has been shown to play important roles in various cancer-related biological processes such as cell proliferation, invasion, and metastasis. Understanding ALYREF's functions provides potential therapeutic targets for multiple cancers.
References：
1. Wang, Jing-Zi, Zhu, Wei, Han, Jie, Wei, Ji-Fu, Yang, Haiwei. 2021. The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer. In Cancer communications (London, England), 41, 560-575. doi:10.1002/cac2.12158. https://pubmed.ncbi.nlm.nih.gov/33991457/
2. Yang, Xin, Yang, Ying, Sun, Bao-Fa, Sun, Ying-Pu, Yang, Yun-Gui. 2017. 5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader. In Cell research, 27, 606-625. doi:10.1038/cr.2017.55. https://pubmed.ncbi.nlm.nih.gov/28418038/
3. Zhao, Yan, Xing, Cheng, Peng, Hongling. 2023. ALYREF (Aly/REF export factor): A potential biomarker for predicting cancer occurrence and therapeutic efficacy. In Life sciences, 338, 122372. doi:10.1016/j.lfs.2023.122372. https://pubmed.ncbi.nlm.nih.gov/38135116/
4. Yang, Qiurong, Wang, Maoye, Xu, Jing, Gu, Jianmei, Zhang, Xu. 2023. LINC02159 promotes non-small cell lung cancer progression via ALYREF/YAP1 signaling. In Molecular cancer, 22, 122. doi:10.1186/s12943-023-01814-x. https://pubmed.ncbi.nlm.nih.gov/37537569/
5. Wang, Ning, Chen, Ri-Xin, Deng, Min-Hua, Liu, Zhuo-Wei, Zhou, Fang-Jian. 2023. m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization. In Cell death & disease, 14, 139. doi:10.1038/s41419-023-05661-y. https://pubmed.ncbi.nlm.nih.gov/36806253/
6. Zhong, Longhua, Wu, Jingxun, Zhou, Bingqian, Ye, Feng, Lin, Xiaoting. 2024. ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export. In NPJ precision oncology, 8, 243. doi:10.1038/s41698-024-00737-0. https://pubmed.ncbi.nlm.nih.gov/39455812/
7. Jin, Yanan, Yao, Jijin, Fu, Jianchang, Xia, Tianliang, Xia, Liangping. 2024. ALYREF promotes the metastasis of nasopharyngeal carcinoma by increasing the stability of NOTCH1 mRNA. In Cell death & disease, 15, 578. doi:10.1038/s41419-024-06959-1. https://pubmed.ncbi.nlm.nih.gov/39117671/
8. Jeong, Su-Jin, Oh, Ji Hoon, Cho, Je-Yoel. 2024. ALYREF enhances breast cancer progression by regulating EZH2. In Heliyon, 10, e37749. doi:10.1016/j.heliyon.2024.e37749. https://pubmed.ncbi.nlm.nih.gov/39386827/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen