C57BL/6JCya-Tertem1flox/Cya
Common Name:
Tert-flox
Product ID:
S-CKO-06159
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tert-flox
Strain ID
CKOCMP-21752-Tert-B6J-VA
Gene Name
Product ID
S-CKO-06159
Gene Alias
EST2; TCS1; TP2; TR; TRT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tertem1flox/Cya mice (Catalog S-CKO-06159) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022104
NCBI RefSeq
NM_009354
Target Region
Exon 3~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Telomerase reverse transcriptase (TERT) is the catalytic subunit of telomerase. Telomerase, an RNA-dependent DNA polymerase, plays a crucial role in maintaining telomere length, which is essential for cells to overcome senescence induced by telomere shortening during DNA replication [5,7]. Telomerase activity is tightly regulated, and TERT expression is repressed in most differentiated cells but can be reactivated in cancer cells, contributing to tumorigenesis through telomere-dependent and-independent mechanisms [1,4,6].
TERT promoter mutations are highly recurrent in over 50 cancer types and are the most common mutation in many cancers [2]. These mutations upregulate TERT transcription, enabling tumor cells to achieve unlimited proliferative capacity by elongating telomeres [3]. In differentiated thyroid carcinoma, TERT-p mutations are associated with high-risk clinicopathological features and worse prognosis [3]. In CNS tumors like meningiomas, medulloblastomas, and primary glial neoplasms, TERT promoter mutations lead to up-regulation of TERT transcription, while in ependymomas and pediatric brain tumors, TERT promoter hypermethylation has the same effect [5]. In melanoma, TERT-p mutations are frequent in chronic and non-chronic sun-damage melanoma and correlate with adverse prognosis, and in acral melanoma, TERT copy number gains and gene amplification predict reduced survival [8]. In urothelial carcinomas, TERT promoter mutations and aberrant methylation play important roles in TERT transcription activation and telomerase activation, and have potential in non-invasive diagnostics, recurrence monitoring, and outcome prediction [9].
In conclusion, TERT is essential for telomere maintenance and its dysregulation, often through promoter mutations or epigenetic changes, is a key event in cancer development across multiple cancer types. The study of TERT in cancer, especially through the identification of its promoter mutations in various malignancies, provides insights into tumorigenesis mechanisms and potential biomarkers for diagnosis, prognosis, and therapeutic targets.
References:
1. Dratwa, Marta, Wysoczańska, Barbara, Łacina, Piotr, Kubik, Tomasz, Bogunia-Kubik, Katarzyna. 2020. TERT-Regulation and Roles in Cancer Formation. In Frontiers in immunology, 11, 589929. doi:10.3389/fimmu.2020.589929. https://pubmed.ncbi.nlm.nih.gov/33329574/
2. Bell, Robert J A, Rube, H Tomas, Xavier-Magalhães, Ana, Song, Jun S, Costello, Joseph F. 2016. Understanding TERT Promoter Mutations: A Common Path to Immortality. In Molecular cancer research : MCR, 14, 315-23. doi:10.1158/1541-7786.MCR-16-0003. https://pubmed.ncbi.nlm.nih.gov/26941407/
3. Matsuse, Michiko, Mitsutake, Norisato. 2023. TERT promoter mutations in thyroid cancer. In Endocrine journal, 70, 1035-1049. doi:10.1507/endocrj.EJ23-0136. https://pubmed.ncbi.nlm.nih.gov/37532521/
4. Liu, Mingdi, Zhang, Yuning, Jian, Yongping, Wang, Yishu, Xu, Zhi-Xiang. 2024. The regulations of telomerase reverse transcriptase (TERT) in cancer. In Cell death & disease, 15, 90. doi:10.1038/s41419-024-06454-7. https://pubmed.ncbi.nlm.nih.gov/38278800/
5. Patel, Bhuvic, Taiwo, Rukayat, Kim, Albert H, Dunn, Gavin P. 2020. TERT, a promoter of CNS malignancies. In Neuro-oncology advances, 2, vdaa025. doi:10.1093/noajnl/vdaa025. https://pubmed.ncbi.nlm.nih.gov/32226942/
6. Song, Young Shin, Park, Young Joo. 2020. Mechanisms of TERT Reactivation and Its Interaction with BRAFV600E. In Endocrinology and metabolism (Seoul, Korea), 35, 515-525. doi:10.3803/EnM.2020.304. https://pubmed.ncbi.nlm.nih.gov/32981294/
7. Heidenreich, Barbara, Kumar, Rajiv. 2016. TERT promoter mutations in telomere biology. In Mutation research. Reviews in mutation research, 771, 15-31. doi:10.1016/j.mrrev.2016.11.002. https://pubmed.ncbi.nlm.nih.gov/28342451/
8. Motaparthi, Kiran, Kim, Jinah, Andea, Aleodor A, Fung, Maxwell A, Emanuel, Patrick O. 2020. TERT and TERT promoter in melanocytic neoplasms: Current concepts in pathogenesis, diagnosis, and prognosis. In Journal of cutaneous pathology, 47, 710-719. doi:10.1111/cup.13691. https://pubmed.ncbi.nlm.nih.gov/32202662/
9. Liu, Tiantian, Li, Shihong, Xia, Chuanyou, Xu, Dawei. 2023. TERT promoter mutations and methylation for telomerase activation in urothelial carcinomas: New mechanistic insights and clinical significance. In Frontiers in immunology, 13, 1071390. doi:10.3389/fimmu.2022.1071390. https://pubmed.ncbi.nlm.nih.gov/36713366/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen