C57BL/6JCya-Tgfb1em1flox/Cya
Common Name
Tgfb1-flox
Product ID
S-CKO-06225
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-21803-Tgfb1-B6J-VA
When using this mouse strain in a publication, please cite “Tgfb1-flox Mouse (Catalog S-CKO-06225) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tgfb1-flox
Strain ID
CKOCMP-21803-Tgfb1-B6J-VA
Gene Name
Product ID
S-CKO-06225
Gene Alias
Tgfb, Tgfb-1, TGFbeta1, TGF-beta1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000002678
NCBI RefSeq
NM_011577
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Overview of Gene Research
Tgfb1, which encodes TGF-β1, is a gene of great significance. TGF-β1 is a potent cytokine that regulates various cellular processes such as immune responses, and is involved in pathways like the TGFB1/INHBA Homodimer/Nodal-SMAD2/3 signaling network. It plays a context-dependent role in cancers and is crucial for normal physiological functions as well as disease-related processes [2,3].
In the context of intestinal regeneration, irradiation-induced damage to intestinal crypts leads to a surge in Tgfb1 expression mediated by monocyte/macrophage cells. Depletion of macrophages or genetic disruption of TGFB signaling significantly impairs the regenerative response. Tgfb1 treatment in organoid culture can induce a fetal-like/regenerative state, and mesenchymal cells' response to Tgfb1 enhances the regenerative response. Pro-regenerative factors YAP/TEAD and SOX9 are activated in the epithelium exposed to Tgfb1 [1].
In pancreatic cancer, the TGFB1/INHBA homodimer/Nodal-SMAD2/3 signaling network is a pivotal molecular target in the development of chemoresistance, regulating mechanisms like enhancing the stem-cell-like properties of pancreatic cancer cells [2].
In conclusion, Tgfb1 is essential for regulating multiple cellular functions. Studies using genetic disruption models in the intestine and pancreatic cancer research have revealed its role in tissue regeneration and cancer-related chemoresistance. Understanding Tgfb1 provides insights into normal physiological repair mechanisms and potential therapeutic targets for diseases such as intestinal injury and pancreatic cancer [1,2].
References：
1. Chen, Lei, Qiu, Xia, Dupre, Abigail, Spence, Jason R, Verzi, Michael P. 2023. TGFB1 induces fetal reprogramming and enhances intestinal regeneration. In Cell stem cell, 30, 1520-1537.e8. doi:10.1016/j.stem.2023.09.015. https://pubmed.ncbi.nlm.nih.gov/37865088/
2. Abdel Mouti, Mai, Pauklin, Siim. 2021. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. In Molecular therapy : the journal of the American Society of Gene Therapy, 29, 920-936. doi:10.1016/j.ymthe.2021.01.002. https://pubmed.ncbi.nlm.nih.gov/33429081/
3. Wang, Cui-Zhu, Zhang, Zi-Qi, Zhang, Yan, Sha, Suo, Xu, Zi-Jun. 2023. Comprehensive characterization of TGFB1 across hematological malignancies. In Scientific reports, 13, 19107. doi:10.1038/s41598-023-46552-8. https://pubmed.ncbi.nlm.nih.gov/37925591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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