C57BL/6JCya-Tgfbr3em1flox/Cya
Common Name:
Tgfbr3-flox
Product ID:
S-CKO-06242
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tgfbr3-flox
Strain ID
CKOCMP-21814-Tgfbr3-B6J-VB
Gene Name
Product ID
S-CKO-06242
Gene Alias
1110036H20Rik; TBRIII
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfbr3em1flox/Cya mice (Catalog S-CKO-06242) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031224
NCBI RefSeq
NM_011578
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Tgfbr3, also known as betaglycan, is a co-receptor in the TGF-β signaling pathway. The TGF-β superfamily signaling pathways are involved in embryonic development, maintaining cellular homeostasis, and regulating immune function [1,2,3,4,5,6,7,9]. Tgfbr3 can regulate the actions of diverse TGF-β ligands [8].
In various cancers, Tgfbr3 often acts as a tumor suppressor gene. In lung cancer, its expression decreases, and the invasion, metastasis, angiogenesis, and apoptosis of lung cancer cells are closely related to its expression [1]. In breast cancer, loss of Tgfbr3 is progressive, and it supports anoikis through suppressing ATF4 signaling [2]. In papillary thyroid cancer, Tgfbr3 inhibits tumor progression by inhibiting the PI3K/AKT pathway and EMT [3]. However, in oesophageal squamous cell carcinoma, TGFBR3 is upregulated and is an independent unfavourable prognostic marker [4]. In addition, in thyroid-eye disease, the miR-103a-3p/TGFBR3 axis regulates TGF-β-induced orbital fibroblast activation and fibrosis [5]. In eosinophilic nasal polyps, MEX3B inhibits collagen production by downregulating epithelial cell Tgfbr3 mRNA stability [6]. In cardiomyocytes, the let-7-Tgfbr3-p38 MAPK signalling plays an important role in apoptosis after infarction [9].
In conclusion, Tgfbr3 is a crucial co-receptor in the TGF-β signaling pathway, significantly influencing various biological processes and disease conditions, especially in multiple types of cancers. Although there is no mention of KO/CKO mouse models in these references, the studies on Tgfbr3 in different diseases help to understand its biological functions and potential as a therapeutic target.
References:
1. Deng, Xin, Ma, Nuoya, He, Junyu, Xu, Fei, Zou, Guoying. . The Role of TGFBR3 in the Development of Lung Cancer. In Protein and peptide letters, 31, 491-503. doi:10.2174/0109298665315841240731060636. https://pubmed.ncbi.nlm.nih.gov/39092729/
2. Hsu, Yu-Jhen, Yin, Yih-Jia, Tsai, Kai-Feng, Hsu, Chien-Yu, Wang, Chun-Chao. 2022. TGFBR3 supports anoikis through suppressing ATF4 signaling. In Journal of cell science, 135, . doi:10.1242/jcs.258396. https://pubmed.ncbi.nlm.nih.gov/35912788/
3. Zhang, Hanrong, Chen, Junxin, Chen, Xin, Guan, Hongyu, Li, Hai. 2024. TGFBR3 inhibits progression of papillary thyroid cancer by inhibiting the PI3K/AKT pathway and EMT. In Endocrine connections, 13, . doi:10.1530/EC-24-0270. https://pubmed.ncbi.nlm.nih.gov/39404708/
4. Zhang, Xueyan, Chen, Yanan, Li, Zhihang, Han, Xueying, Liang, Yingying. 2020. TGFBR3 is an independent unfavourable prognostic marker in oesophageal squamous cell cancer and is positively correlated with Ki-67. In International journal of experimental pathology, 101, 223-229. doi:10.1111/iep.12380. https://pubmed.ncbi.nlm.nih.gov/33146446/
5. Xie, Bingyu, Xiong, Wei, Zhang, Feng, Ma, Chen, Chen, Haiyan. 2022. The miR-103a-3p/TGFBR3 axis regulates TGF-β-induced orbital fibroblast activation and fibrosis in thyroid-eye disease. In Molecular and cellular endocrinology, 559, 111780. doi:10.1016/j.mce.2022.111780. https://pubmed.ncbi.nlm.nih.gov/36179941/
6. Liu, Jin-Xin, Chen, Ao-Nan, Yu, Qihong, Liao, Bo, Liu, Zheng. 2023. MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability. In JCI insight, 8, . doi:10.1172/jci.insight.159058. https://pubmed.ncbi.nlm.nih.gov/36976645/
7. Caza, Tiffany N, Hassen, Samar I, Kenan, Daniel J, Beck, Laurence H, Larsen, Christopher P. 2021. Transforming Growth Factor Beta Receptor 3 (TGFBR3)-Associated Membranous Nephropathy. In Kidney360, 2, 1275-1286. doi:10.34067/KID.0001492021. https://pubmed.ncbi.nlm.nih.gov/35369660/
8. Li, Yining, Lin, Yeu-Farn, Zhou, Xiang, Clarke, Hugh J, Bernard, Daniel J. . Ablation of TGFBR3 (betaglycan) in oocytes does not affect fertility in female mice. In Reproduction (Cambridge, England), 161, 289-294. doi:10.1530/REP-20-0515. https://pubmed.ncbi.nlm.nih.gov/33300886/
9. Chen, Chen-Yun, Choong, Oi Kuan, Liu, Li-Wei, Kamp, Timothy J, Hsieh, Patrick C H. 2019. MicroRNA let-7-TGFBR3 signalling regulates cardiomyocyte apoptosis after infarction. In EBioMedicine, 46, 236-247. doi:10.1016/j.ebiom.2019.08.001. https://pubmed.ncbi.nlm.nih.gov/31401194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen