C57BL/6JCya-Thpoem1flox/Cya
Common Name:
Thpo-flox
Product ID:
S-CKO-06268
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Thpo-flox
Strain ID
CKOCMP-21832-Thpo-B6J-VA
Gene Name
Product ID
S-CKO-06268
Gene Alias
Mgdf; Ml; Mpllg; Tpo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Thpoem1flox/Cya mice (Catalog S-CKO-06268) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115437
NCBI RefSeq
NM_009379
Target Region
Exon 5~6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Thpo, also known as thrombopoietin, is an essential cytokine for hematopoietic stem cell (HSC) maintenance and megakaryocyte differentiation [4]. It functions within the THPO-MPL pathway, which is crucial for normal hematopoiesis [1]. Disturbances in this pathway can lead to various bone marrow-related disorders. Genetic models, such as zebrafish models, can be valuable tools for studying Thpo [5].
Germline mutations of Thpo have been reported as causes of hereditary thrombocythemia. These mutations lead to increased production of Thpo protein through mechanisms like exon 3 skipping or base deletions that affect upstream suppressive open reading frames [2]. On the other hand, monoallelic loss-of-function Thpo variants cause heritable thrombocytopenia [3]. A zebrafish Thpo-deficient model showed that disruption of Thpo led to reduced Thpo expression and thrombocytopenia, and was used to study human clinical Thpo variants and evaluate Thpo-receptor agonists [5].
In conclusion, Thpo plays a vital role in hematopoiesis, especially in platelet production. Studies using models like the Thpo-deficient zebrafish have revealed its role in thrombocytopenia and thrombocythemia. Understanding Thpo's functions through these models provides insights into the mechanisms of these blood-related diseases, potentially guiding future diagnostic and therapeutic strategies.
References:
1. Dasouki, Majed, Saadi, Irfan, Ahmed, Syed O. 2014. THPO-MPL pathway and bone marrow failure. In Hematology/oncology and stem cell therapy, 8, 6-9. doi:10.1016/j.hemonc.2014.11.005. https://pubmed.ncbi.nlm.nih.gov/25482588/
2. Kimura, Hiroyuki, Onozawa, Masahiro, Matsukawa, Toshihiro, Kondo, Takeshi, Teshima, Takanori. 2024. Relative impact of THPO mutation causing hereditary thrombocythemia. In Experimental hematology, 134, 104208. doi:10.1016/j.exphem.2024.104208. https://pubmed.ncbi.nlm.nih.gov/38548144/
3. Cornish, Naomi, Aungraheeta, M Riyaad, FitzGibbon, Lucy, Turro, Ernest, Mumford, Andrew D. . Monoallelic loss-of-function THPO variants cause heritable thrombocytopenia. In Blood advances, 4, 920-924. doi:10.1182/bloodadvances.2019001293. https://pubmed.ncbi.nlm.nih.gov/32150607/
4. Tsutsumi, Naotaka, Masoumi, Zahra, James, Sophie C, Hitchcock, Ian S, Garcia, K Christopher. 2023. Structure of the thrombopoietin-MPL receptor complex is a blueprint for biasing hematopoiesis. In Cell, 186, 4189-4203.e22. doi:10.1016/j.cell.2023.07.037. https://pubmed.ncbi.nlm.nih.gov/37633268/
5. Yang, Lian, Wu, Liangliang, Meng, Panpan, Lin, Qing, Zhang, Yiyue. 2022. Generation of a thrombopoietin-deficient thrombocytopenia model in zebrafish. In Journal of thrombosis and haemostasis : JTH, 20, 1900-1909. doi:10.1111/jth.15772. https://pubmed.ncbi.nlm.nih.gov/35622056/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen