C57BL/6JCya-Trim24em1flox/Cya
Common Name:
Trim24-flox
Product ID:
S-CKO-06290
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trim24-flox
Strain ID
CKOCMP-21848-Trim24-B6J-VA
Gene Name
Product ID
S-CKO-06290
Gene Alias
A130082H20Rik; D430004I05Rik; TIF1; TIF1-alpha; TIF1alpha; Tif1a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim24em1flox/Cya mice (Catalog S-CKO-06290) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031859
NCBI RefSeq
NM_145076
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Trim24, also known as TRIpartite motif-containing protein 24, is an E3 ligase and epigenetic regulator involved in multiple biological processes. It participates in pathways such as the STAT6-related macrophage polarization regulation, DNA double-strand break repair, and insulin-related mRNA stability regulation. It has significance in diseases including cancer, allergic rhinitis, and HIV-1 latency [1,3,4,5,6].
In macrophage polarization, Trim24-knockout (KO) in both mouse and human macrophages promotes M2 polarization potentially compromising antitumor immune responses, as Trim24 promotes Stat6 acetylation to suppress M2 polarization [1]. In nasopharyngeal carcinoma, depletion of TRIM24 inhibits M1 macrophage polarization, facilitating NPC cell growth and migration [2]. In DNA double-strand breaks, TRIM24-depleted human hepatocellular carcinoma cells are more sensitive to cancer therapy-induced apoptosis and show retarded tumor growth in a xenograft model, as TRIM24 is essential for MRN complex recruitment [3]. In allergic rhinitis, TRIM24-conditional knockout (CKO) mice in CD4+ T cells have more severe AR symptoms with enhanced Th2 polarization due to decreased STAT6 acetylation [5].
In conclusion, Trim24 KO/CKO mouse models have revealed its crucial roles in macrophage polarization, DNA repair, and allergic rhinitis. Trim24 is important in regulating these biological processes, and its dysregulation is associated with tumor progression and allergic diseases, providing potential therapeutic targets.
References:
1. Yu, Tao, Gan, Shucheng, Zhu, Qingchen, Qin, Jun, Xiao, Yichuan. 2019. Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24. In Nature communications, 10, 4353. doi:10.1038/s41467-019-12384-2. https://pubmed.ncbi.nlm.nih.gov/31554795/
2. Liu, Huai, Tang, Ling, Gong, Sha, Wang, Hui, Chen, Pan. 2023. USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24. In Cell death & disease, 14, 852. doi:10.1038/s41419-023-06368-w. https://pubmed.ncbi.nlm.nih.gov/38129408/
3. Wang, Ya, Yao, Yuanbing, Wei, Qunhui, Wan, Fengyi, Fu, Kai. 2022. TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex. In Oncogene, 42, 586-600. doi:10.1038/s41388-022-02580-8. https://pubmed.ncbi.nlm.nih.gov/36550358/
4. Wei, Wen, Chen, Qiaoli, Liu, Minjun, Wang, Hong-Yu, Chen, Shuai. 2022. TRIM24 is an insulin-responsive regulator of P-bodies. In Nature communications, 13, 3972. doi:10.1038/s41467-022-31735-0. https://pubmed.ncbi.nlm.nih.gov/35803934/
5. Yue, Liyan, Jia, Qiaojing, Dong, Jinhui, Ren, Xiumin, Xu, Ou. . TRIM24-Mediated Acetylation of STAT6 Suppresses Th2-Induced Allergic Rhinitis. In Allergy, asthma & immunology research, 15, 603-613. doi:10.4168/aair.2023.15.5.603. https://pubmed.ncbi.nlm.nih.gov/37827979/
6. Horvath, Riley M, Dahabieh, Matthew, Malcolm, Tom, Sadowski, Ivan. 2023. TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation. In Communications biology, 6, 86. doi:10.1038/s42003-023-04484-z. https://pubmed.ncbi.nlm.nih.gov/36690785/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen