C57BL/6JCya-Ccnoem1flox/Cya
Common Name:
Ccno-flox
Product ID:
S-CKO-06308
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccno-flox
Strain ID
CKOCMP-218630-Ccno-B6J-VA
Gene Name
Product ID
S-CKO-06308
Gene Alias
Ccnu; E130318K11; UDG2; Ung2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccnoem1flox/Cya mice (Catalog S-CKO-06308) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038404
NCBI RefSeq
NM_001081062
Target Region
Exon 2~3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ccno, also known as Cyclin O, is a gene that plays a crucial role in regulating the cell cycle. It is involved in the process of multiciliogenesis, which is essential for the formation of multiple motile cilia. These cilia are important for mucociliary clearance and fluid transport along epithelial surfaces [1,2,4,5,6].
In mouse models, the genetic deletion of Ccno leads to a reduced number of multiple motile cilia. This is caused by compromised generation of centrioles at deuterosomes, which are the major amplification platform for centrioles in multiciliated cells (MCCs). Ccno -deficient MCCs fail to sufficiently generate deuterosomes, resulting in only a reduced number of fully functional centrioles that can mature into ciliary basal bodies. These mice also exhibit characteristic phenotypes of MCC dysfunction, such as severe hydrocephalus and mucociliary clearance deficits [6].
In conclusion, Ccno is a key regulator in the transcriptional regulation of multiciliogenesis. Its deficiency in mouse models reveals its importance in the formation of motile cilia and associated physiological functions. Mutations in Ccno in humans are linked to primary ciliary dyskinesia (PCD), a genetically heterogeneous disorder characterized by abnormal ciliary motility, which can lead to severe respiratory diseases, infertility, and other clinical manifestations [1,2,3,4,5].
References:
1. Wang, Lina, Gao, Liwei, Chen, Yinghong, Xu, BaoPing. 2024. Transcriptional regulation of CCNO during the formation of multiple motile cilia. In Biochemical and biophysical research communications, 735, 150428. doi:10.1016/j.bbrc.2024.150428. https://pubmed.ncbi.nlm.nih.gov/39094231/
2. Zhang, Yun-Yan, Lou, Yan, Yan, Han, Tang, Hao. . CCNO mutation as a cause of primary ciliary dyskinesia: A case report. In World journal of clinical cases, 10, 9148-9155. doi:10.12998/wjcc.v10.i25.9148. https://pubmed.ncbi.nlm.nih.gov/36157652/
3. Guan, Yuhong, Yang, Haiming, Yao, Xingfeng, Ge, Wentong, Ni, Xin. 2021. Clinical and Genetic Spectrum of Children With Primary Ciliary Dyskinesia in China. In Chest, 159, 1768-1781. doi:10.1016/j.chest.2021.02.006. https://pubmed.ncbi.nlm.nih.gov/33577779/
4. Henriques, Ana Raquel, Constant, Carolina, Descalço, Andreia, Pereira, Luísa, Bandeira, Teresa. 2021. Primary ciliary dyskinesia due to CCNO mutations-A genotype-phenotype correlation contribution. In Pediatric pulmonology, 56, 2776-2779. doi:10.1002/ppul.25440. https://pubmed.ncbi.nlm.nih.gov/34102041/
5. Ma, Cong, Wu, Huan, Zhu, Damin, Cao, Yunxia, Xu, Xiaofeng. 2021. Bi-allelic mutations in MCIDAS and CCNO cause human infertility associated with abnormal gamete transport. In Clinical genetics, 100, 731-742. doi:10.1111/cge.14067. https://pubmed.ncbi.nlm.nih.gov/34569065/
6. Funk, Maja C, Bera, Agata N, Menchen, Tabea, Frank, Marcus, Arnold, Sebastian J. 2015. Cyclin O (Ccno) functions during deuterosome-mediated centriole amplification of multiciliated cells. In The EMBO journal, 34, 1078-89. doi:10.15252/embj.201490805. https://pubmed.ncbi.nlm.nih.gov/25712475/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen