C57BL/6JCya-Cd27em1flox/Cya
Common Name:
Cd27-flox
Product ID:
S-CKO-06391
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cd27-flox
Strain ID
CKOCMP-21940-Cd27-B6J-VA
Gene Name
Product ID
S-CKO-06391
Gene Alias
S152; Tnfrsf7; Tp55
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd27em1flox/Cya mice (Catalog S-CKO-06391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032486
NCBI RefSeq
NM_001033126
Target Region
Exon 1~2
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Cd27, a member of the tumour necrosis factor receptor superfamily (TNFRSF7), plays a key role in T-cell activation by providing a costimulatory signal. Its ligation with CD70 enhances T-cell proliferation and differentiation to effector and memory T cells. It is also constitutively expressed on a broad range of T-cells (αβ and γδ), NK-cells and B-cells, and involved in regulating lymphocyte function [2,3]. The CD27-CD70 pathway is associated with multiple biological processes and disease conditions, including anti-viral immunity, cancer, and autoimmune diseases [2,3,4]. Genetic models, such as KO/CKO mouse models, can be valuable for studying its functions.
In naive CD8+ T cells, CD27 ligation by trimeric CD70 ligand triggers internalization and degradation, recruiting TRAF2 and SHP-1, and modulating TCR and CD28 signals to promote memory-associated gene regulatory networks, which is relevant to T-cell immunotherapy [1]. In mice, co-stimulation of CD8+ T-cells through CD27 promotes immune activation and enhances primary, secondary, memory and recall responses towards viral infections [3]. Blockade of the CD27-CD70 pathway has been shown to ameliorate disease manifestations in animal models of autoimmune diseases, like murine collagen-induced arthritis and experimental colitis [4].
In conclusion, Cd27 is crucial for T-cell activation, differentiation, and immune responses. Studies using KO/CKO mouse models have revealed its role in anti-viral immunity and autoimmune diseases, suggesting that targeting the CD27-CD70 pathway could be a potential therapeutic strategy for these disease areas.
References:
1. Jaeger-Ruckstuhl, Carla A, Lo, Yun, Fulton, Elena, Furlan, Scott N, Riddell, Stanley R. . Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell activation promotes memory-associated gene regulatory networks. In Immunity, 57, 287-302.e12. doi:10.1016/j.immuni.2024.01.011. https://pubmed.ncbi.nlm.nih.gov/38354704/
2. Starzer, Angelika M, Berghoff, Anna S. . New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). In ESMO open, 4, e000629. doi:10.1136/esmoopen-2019-000629. https://pubmed.ncbi.nlm.nih.gov/32152062/
3. Grant, Emma J, Nüssing, Simone, Sant, Sneha, Clemens, E Bridie, Kedzierska, Katherine. 2017. The role of CD27 in anti-viral T-cell immunity. In Current opinion in virology, 22, 77-88. doi:10.1016/j.coviro.2016.12.001. https://pubmed.ncbi.nlm.nih.gov/28086150/
4. Han, Bobby Kwanghoon, Olsen, Nancy J, Bottaro, Andrea. 2015. The CD27-CD70 pathway and pathogenesis of autoimmune disease. In Seminars in arthritis and rheumatism, 45, 496-501. doi:10.1016/j.semarthrit.2015.08.001. https://pubmed.ncbi.nlm.nih.gov/26359318/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen