C57BL/6JCya-Tuba3aem1flox/Cya
Common Name:
Tuba3a-flox
Product ID:
S-CKO-06485
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tuba3a-flox
Strain ID
CKOCMP-22144-Tuba3a-B6J-VA
Gene Name
Product ID
S-CKO-06485
Gene Alias
M[a]3; Tuba3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tuba3aem1flox/Cya mice (Catalog S-CKO-06485) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000088246
NCBI RefSeq
NM_009446
Target Region
Exon 2~4
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Tuba3a, a gene with yet-to-be-detailed aliases, is essential for male germ cell development [1]. It may also be involved in pathways related to early embryo development and retinal development. Its biological importance lies in its role in maintaining normal cellular functions in specific tissues. Genetic models, such as knockout (KO) mice, are valuable tools for studying Tuba3a's functions.
In KO mouse models, Tuba3a/3b knockout mice completely lost germ cells at the early postnatal stages, indicating its crucial role in male germ cell development [1]. In addition, in conditional knockdown experiments of DNA methyltransferase 1 in mice, increased expression of Tuba3a was detected in mutant retina, which was related to disrupted retinal pigment epithelium morphology and photoreceptor outer segment defects [2].
In conclusion, Tuba3a is critical for male germ cell development and may also play a role in retinal development. The KO mouse models have significantly contributed to understanding Tuba3a's functions in these biological processes, potentially providing insights into related male reproductive and retinal diseases.
References：
1. Akter, Most Sumona, Hada, Masashi, Shikata, Daiki, Ogura, Atsuo, Matoba, Shogo. 2021. Nuclease technology-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice. In Scientific reports, 11, 15438. doi:10.1038/s41598-021-94851-9. https://pubmed.ncbi.nlm.nih.gov/34326397/
2. Nasonkin, Igor O, Merbs, Shannath L, Lazo, Kevin, Rodriguez-Boulan, Enrique J, Swaroop, Anand. 2013. Conditional knockdown of DNA methyltransferase 1 reveals a key role of retinal pigment epithelium integrity in photoreceptor outer segment morphogenesis. In Development (Cambridge, England), 140, 1330-41. doi:10.1242/dev.086603. https://pubmed.ncbi.nlm.nih.gov/23406904/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen