C57BL/6JCya-Ufd1em1flox/Cya
Common Name
Ufd1-flox
Product ID
S-CKO-06531
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22230-Ufd1-B6J-VA
When using this mouse strain in a publication, please cite “Ufd1-flox Mouse (Catalog S-CKO-06531) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ufd1-flox
Strain ID
CKOCMP-22230-Ufd1-B6J-VA
Gene Name
Product ID
S-CKO-06531
Gene Alias
Ufd1l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000005394
NCBI RefSeq
NM_011672
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Overview of Gene Research
Ufd1, the ubiquitin fusion degradation 1, is an essential component in the ubiquitin-proteasome pathway. It functions as a cofactor in the Cdc48-Ufd1-Npl4 complex, which is crucial for recognizing polyubiquitin-tagged proteins and facilitating their presentation to the 26S proteasome for degradation [1]. This complex is involved in eukaryotic cellular processes, such as the clearance of polyubiquitylated targets [2].
In zebrafish, allelic loss of ufd1 induces tumor cell apoptosis and impairs MYC-driven T-cell acute lymphoblastic leukemia (T-ALL) progression, suggesting Ufd1 contributes to MYC-mediated leukemia aggressiveness through suppression of the proapoptotic unfolded protein response [3]. In Enterovirus A71 (EVA71) infection, knockdown of Ufd1 significantly decreased the binding and entry of EVA71 to host cells, indicating its role in the viral entry process [4].
In conclusion, Ufd1 is vital in the ubiquitin-proteasome pathway, participating in crucial cellular processes. Its functions have been revealed through genetic models like zebrafish, highlighting its significance in leukemia and viral entry-related disease areas. Understanding Ufd1 can potentially provide new insights into the mechanisms of these diseases and offer new strategies for treatment.
References：
1. Bays, Nathan W, Hampton, Randolph Y. . Cdc48-Ufd1-Npl4: stuck in the middle with Ub. In Current biology : CB, 12, R366-71. doi:. https://pubmed.ncbi.nlm.nih.gov/12015140/
2. Lee, Hyein G, Lemmon, Abigail A, Lima, Christopher D. 2022. SUMO enhances unfolding of SUMO-polyubiquitin-modified substrates by the Ufd1/Npl4/Cdc48 complex. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2213703120. doi:10.1073/pnas.2213703120. https://pubmed.ncbi.nlm.nih.gov/36574706/
3. Huiting, L N, Samaha, Y, Zhang, G L, Kelliher, M A, Feng, H. 2018. UFD1 contributes to MYC-mediated leukemia aggressiveness through suppression of the proapoptotic unfolded protein response. In Leukemia, 32, 2339-2351. doi:10.1038/s41375-018-0141-x. https://pubmed.ncbi.nlm.nih.gov/29743725/
4. Yan, Jingjing, Wang, Meng, Wang, Min, Yi, Zhigang, Zhang, Shuye. 2020. Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species. In Virus research, 283, 197974. doi:10.1016/j.virusres.2020.197974. https://pubmed.ncbi.nlm.nih.gov/32289342/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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