C57BL/6JCya-Vamp2em1flox/Cya
Common Name
Vamp2-flox
Product ID
S-CKO-06575
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22318-Vamp2-B6J-VA
When using this mouse strain in a publication, please cite “Vamp2-flox Mouse (Catalog S-CKO-06575) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Vamp2-flox
Strain ID
CKOCMP-22318-Vamp2-B6J-VA
Gene Name
Product ID
S-CKO-06575
Gene Alias
Syb-2, Syb2, Vamp-2, sybII
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021273
NCBI RefSeq
NM_009497
Target Region
Exon 3~5
Size of Effective Region
~2.9 kb
Overview of Gene Research
Vamp2, also known as vesicle-associated membrane protein 2, is a component of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex [2,4,5,6,7]. It plays a crucial role in vesicular trafficking and membrane fusion processes, which are vital for various biological functions such as neurotransmitter release, epidermal differentiation, and myelin formation [3,4,5,6]. It is also involved in pathways related to synaptic physiology and pathophysiology, as well as cellular autophagy [1,2,3]. Genetic models, especially KO/CKO mouse models, are valuable for studying Vamp2's function.
In murine studies, deletion of Vamp2 leads to aberrant skin stratification and enucleation, indicating its importance in epidermal differentiation and carcinogenesis [3]. Genetic inactivation of Vamp2/3 in myelinating oligodendrocytes causes severe hypomyelination and premature death, highlighting its role in CNS myelination [4]. Toxin-mediated cleavage of VAMP2/3 in the OL lineage of mice impairs the maturation of early oligodendrocytes into myelinating ones, further emphasizing its role in myelin formation [6].
In conclusion, Vamp2 is essential for processes like epidermal differentiation, CNS myelination, and oligodendrocyte maturation as revealed through mouse model-based research. These findings contribute to our understanding of diseases related to skin development, neurodegeneration, and demyelinating disorders.
References：
1. Agarwal, Aishwarya, Chandran, Aswathy, Raza, Farheen, Warren, Alan J, Lautenschläger, Janin. 2024. VAMP2 regulates phase separation of α-synuclein. In Nature cell biology, 26, 1296-1308. doi:10.1038/s41556-024-01451-6. https://pubmed.ncbi.nlm.nih.gov/38951707/
2. Wang, Chuchu, Zhang, Kai, Cai, Bin, Li, Dan, Diao, Jiajie. 2024. VAMP2 chaperones α-synuclein in synaptic vesicle co-condensates. In Nature cell biology, 26, 1287-1295. doi:10.1038/s41556-024-01456-1. https://pubmed.ncbi.nlm.nih.gov/38951706/
3. Liu, Han, Su, Peihong, Li, Yuanyuan, Tan, Minjia, Wu, Xiaoyang. 2024. VAMP2 controls murine epidermal differentiation and carcinogenesis by regulation of nucleophagy. In Developmental cell, 59, 2005-2016.e4. doi:10.1016/j.devcel.2024.05.004. https://pubmed.ncbi.nlm.nih.gov/38810653/
4. Lam, Mable, Takeo, Koji, Almeida, Rafael G, Kantarci, Husniye, Zuchero, J Bradley. 2022. CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes. In Nature communications, 13, 5583. doi:10.1038/s41467-022-33200-4. https://pubmed.ncbi.nlm.nih.gov/36151203/
5. Meijer, Marieke, Öttl, Miriam, Yang, Jie, Zhang, Yongli, Verhage, Matthijs. 2024. Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes. In Nature communications, 15, 2652. doi:10.1038/s41467-024-46828-1. https://pubmed.ncbi.nlm.nih.gov/38531902/
6. Fekete, Christopher D, Horning, Robert Z, Doron, Matan S, Nishiyama, Akiko. 2023. Cleavage of VAMP2/3 Affects Oligodendrocyte Lineage Development in the Developing Mouse Spinal Cord. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 43, 6592-6608. doi:10.1523/JNEUROSCI.2206-21.2023. https://pubmed.ncbi.nlm.nih.gov/37620160/
7. Li, Lihua, Wang, Kunpeng, Jia, Rongjun, Mo, Jinggang, Ren, Fu. 2022. Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation. In Redox biology, 56, 102435. doi:10.1016/j.redox.2022.102435. https://pubmed.ncbi.nlm.nih.gov/36029649/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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