C57BL/6JCya-Dapem1flox/Cya
Common Name:
Dap-flox
Product ID:
S-CKO-06603
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dap-flox
Strain ID
CKOCMP-223453-Dap-B6J-VA
Gene Name
Product ID
S-CKO-06603
Gene Alias
4921531N22Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dapem1flox/Cya mice (Catalog S-CKO-06603) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044524
NCBI RefSeq
NM_146057
Target Region
Exon 3~4
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Dap can refer to multiple entities. In the context of genes, DAP-kinase (Death-associated protein kinase) is a calcium/calmodulin-dependent serine/threonine kinase. It participates in various apoptosis systems, and is a tumor suppressor mediating cell death pathways like apoptosis and programmed necrosis. It is also identified as an essential regulator of autophagy, activated by signals such as cytokines and ER stress [1].
Functional studies suggest that DAP-kinase may direct autophagy specifically towards autophagic cell death. It has been mapped to distinct stages in autophagy signaling, including the Beclin-1/phosphatidylinositol 3-kinase (PI(3)K) complex necessary for autophagosome formation, and an interaction with the LC3 binding protein, MAP1B, which may regulate vesicle trafficking [1]. Additionally, DAP-kinase suppresses integrin-mediated cell adhesion and signal transduction, and this adhesion-inhibitory effect accounts for a major mechanism of the apoptosis it induces. It exerts apoptotic effects by suppressing integrin functions and integrin-mediated survival signals, thereby activating a p53-dependent apoptotic pathway [2]. Also, loss of DAP-kinase expression provides a mechanism that links suppression of apoptosis to metastasis. Restoration of DAP-kinase to physiological levels in high-metastatic Lewis carcinoma cells suppressed their ability to form lung metastases and delayed local tumour growth [3].
In conclusion, DAP-kinase is crucial in regulating apoptosis, autophagy, and metastasis. Studies on DAP-kinase, especially through in vivo models like the ones using Lewis carcinoma cells, have revealed its role in cancer-related processes. Understanding DAP-kinase provides insights into the mechanisms of cell death and metastasis, which could potentially lead to new strategies for cancer treatment.
References:
1. Bialik, Shani, Kimchi, Adi. 2009. Lethal weapons: DAP-kinase, autophagy and cell death: DAP-kinase regulates autophagy. In Current opinion in cell biology, 22, 199-205. doi:10.1016/j.ceb.2009.11.004. https://pubmed.ncbi.nlm.nih.gov/20005690/
2. Wang, Won-Jing, Kuo, Jean-Cheng, Yao, Chung-Chen, Chen, Ruey-Hwa. 2002. DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals. In The Journal of cell biology, 159, 169-79. doi:. https://pubmed.ncbi.nlm.nih.gov/12370243/
3. Inbal, B, Cohen, O, Polak-Charcon, S, Eisenbach, L, Kimchi, A. . DAP kinase links the control of apoptosis to metastasis. In Nature, 390, 180-4. doi:. https://pubmed.ncbi.nlm.nih.gov/9367156/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen