C57BL/6JCya-Dyrk3em1flox/Cya
Common Name:
Dyrk3-flox
Product ID:
S-CKO-06940
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dyrk3-flox
Strain ID
CKOCMP-226419-Dyrk3-B6J-VA
Gene Name
Product ID
S-CKO-06940
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dyrk3em1flox/Cya mice (Catalog S-CKO-06940) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016670
NCBI RefSeq
NM_145508
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
DYRK3, short for dual-specificity tyrosine-phosphorylation-regulated kinase 3, is a member of the DYRK family. It plays crucial roles in multiple biological processes. It is involved in pathways such as secretory trafficking, where it maintains the liquid-like state of ER exit sites (ERESs) to enable proper cargo trafficking through the early secretory pathway [1]. It also participates in regulating axonal retrograde transport, neurotransmitter release, cell migration, and is associated with processes like erythropoiesis, stress granule disassembly, and cancer-related pathways [3,4,5,7].
In DYRK3 knockout mice, erythropoiesis is enhanced during anemia, indicating that DYRK3 normally attenuates red cell production in such conditions [7]. In the context of oral squamous cell carcinoma (OSCC), inhibiting DYRK3 can disrupt purinosome formation and influence the survival rate of radiation-resistant OSCC cell lines, suggesting its role in radiotherapy resistance [2]. In glioblastoma, knockdown of DYRK3 inhibits mitochondrial fission, leading to increased oxidative phosphorylation and reduced glycolysis, and impairs cell migration and invasion [6]. In melanoma, DYRK3 knockdown or blockade of its phosphorylation of p62 at Thr-269 inhibits melanoma growth, colony formation, and cell migration [8].
In conclusion, DYRK3 is essential in regulating diverse biological functions including cell trafficking, neuronal functions, and cell growth. Gene knockout models have revealed its significant roles in diseases such as anemia, various cancers including OSCC, glioblastoma, and melanoma. Understanding DYRK3 through these models provides insights into disease mechanisms and potential therapeutic strategies for these conditions.
References:
1. Gallo, Raffaella, Rai, Arpan Kumar, McIntyre, Alexa B R, Meyer, Katrina, Pelkmans, Lucas. 2023. DYRK3 enables secretory trafficking by maintaining the liquid-like state of ER exit sites. In Developmental cell, 58, 1880-1897.e11. doi:10.1016/j.devcel.2023.08.005. https://pubmed.ncbi.nlm.nih.gov/37643612/
2. Huang, Chin-Sheng, Hsieh, Ming-Shou, Yadav, Vijesh Kumar, Yeh, Chi-Tai, Huang, Mao-Suan. 2023. PAICS/DYRK3 Multienzyme Interactions as Coregulators of Purinosome Formation and Metabolism on Radioresistance in Oral Squamous Cell Carcinoma. In International journal of molecular sciences, 24, . doi:10.3390/ijms242417346. https://pubmed.ncbi.nlm.nih.gov/38139175/
3. Lee, Ye Hyung, Suh, Bo Kyoung, Lee, Unghwi, Park, Sang Ki, Chung, Kwang Chul. 2022. DYRK3 phosphorylates SNAPIN to regulate axonal retrograde transport and neurotransmitter release. In Cell death discovery, 8, 503. doi:10.1038/s41420-022-01290-0. https://pubmed.ncbi.nlm.nih.gov/36585413/
4. Ramella, Martina, Ribolla, Lucrezia Maria, Surini, Sara, Pelkmans, Lucas, de Curtis, Ivan. 2024. Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions. In iScience, 27, 109440. doi:10.1016/j.isci.2024.109440. https://pubmed.ncbi.nlm.nih.gov/38510137/
5. Mediani, Laura, Antoniani, Francesco, Galli, Veronica, Alberti, Simon, Carra, Serena. 2021. Hsp90-mediated regulation of DYRK3 couples stress granule disassembly and growth via mTORC1 signaling. In EMBO reports, 22, e51740. doi:10.15252/embr.202051740. https://pubmed.ncbi.nlm.nih.gov/33738926/
6. Kim, Kyeongmin, Lee, Sungmin, Kang, Hyunkoo, Youn, HyeSook, Youn, BuHyun. 2021. Dual Specificity Kinase DYRK3 Promotes Aggressiveness of Glioblastoma by Altering Mitochondrial Morphology and Function. In International journal of molecular sciences, 22, . doi:10.3390/ijms22062982. https://pubmed.ncbi.nlm.nih.gov/33804169/
7. Bogacheva, Olga, Bogachev, Oleg, Menon, Madhu, Erickson-Miller, Connie L, Wojchowski, Don M. 2008. DYRK3 dual-specificity kinase attenuates erythropoiesis during anemia. In The Journal of biological chemistry, 283, 36665-75. doi:10.1074/jbc.M807844200. https://pubmed.ncbi.nlm.nih.gov/18854306/
8. Lee, Ye Hyung, Yoon, A-Rum, Yun, Chae-Ok, Chung, Kwang Chul. 2024. Dual-specificity kinase DYRK3 phosphorylates p62 at the Thr-269 residue and promotes melanoma progression. In The Journal of biological chemistry, 300, 107206. doi:10.1016/j.jbc.2024.107206. https://pubmed.ncbi.nlm.nih.gov/38519031/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen