C57BL/6JCya-Ikzf2em1flox/Cya
Common Name
Ikzf2-flox
Product ID
S-CKO-07125
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22779-Ikzf2-B6J-VA
When using this mouse strain in a publication, please cite “Ikzf2-flox Mouse (Catalog S-CKO-07125) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ikzf2-flox
Strain ID
CKOCMP-22779-Ikzf2-B6J-VA
Gene Name
Product ID
S-CKO-07125
Gene Alias
Helios, Zfpn1a2, Znfn1a2, A730095J18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000027146
NCBI RefSeq
NM_011770
Target Region
Exon 7~8
Size of Effective Region
~4.0 kb
Overview of Gene Research
Ikzf2, also known as Helios, is a transcription factor belonging to the Ikaros family. It plays a crucial role in maintaining the function and stability of regulatory T (Treg) cells, and is involved in multiple biological processes such as T-follicular helper, NK-and T-regulatory cell physiology [1,4]. It may also participate in regulating the development of ICOS+ Th cells to mediate immune response in the spleen of Schistosoma japonicum-infected mice [6].
In animal models, IKZF2 deficiency reduces tumor growth in mice, indicating its potential role in tumor-immune evasion. Specifically, in myeloid leukemia, IKZF2 is highly expressed in leukemic stem cells (LSCs), and its depletion in acute myeloid leukemia (AML) cells reduces colony formation, increases differentiation and apoptosis, and delays leukemogenesis. IKZF2 regulates a HOXA9 self-renewal gene expression program and inhibits a C/EBP-driven differentiation program in MLL-AF9 LSCs [5]. Dual degraders of IKZF2 and casein kinase 1α (CK1α) like DEG-35 and DEG-77 can delay leukemia progression in murine and human AML mouse models by blocking cell growth and inducing myeloid differentiation [2,3]. Also, treatment with the selective IKZF2 glue degrader NVP-DKY709 delays tumor growth in mice with a humanized immune system and enhances immunization responses in cynomolgus monkeys [1].
In conclusion, Ikzf2 is essential for the function and stability of Treg cells and has a significant impact on tumor growth and leukemia development. The study of Ikzf2 knockout or conditional knockout mouse models has provided valuable insights into its role in cancer immunotherapy and leukemia treatment, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Bonazzi, Simone, d'Hennezel, Eva, Beckwith, Rohan E J, Visser, Michael, Solomon, Jonathan M. 2023. Discovery and characterization of a selective IKZF2 glue degrader for cancer immunotherapy. In Cell chemical biology, 30, 235-247.e12. doi:10.1016/j.chembiol.2023.02.005. https://pubmed.ncbi.nlm.nih.gov/36863346/
2. Park, Sun-Mi, Miyamoto, David K, Han, Grace Y Q, Kharas, Michael G, Woo, Christina M. 2023. Dual IKZF2 and CK1α degrader targets acute myeloid leukemia cells. In Cancer cell, 41, 726-739.e11. doi:10.1016/j.ccell.2023.02.010. https://pubmed.ncbi.nlm.nih.gov/36898380/
3. Miyamoto, David K, Curnutt, Nicole M, Park, Sun-Mi, Kharas, Michael G, Woo, Christina M. 2023. Design and Development of IKZF2 and CK1α Dual Degraders. In Journal of medicinal chemistry, 66, 16953-16979. doi:10.1021/acs.jmedchem.3c01736. https://pubmed.ncbi.nlm.nih.gov/38085607/
4. Shahin, Tala, Mayr, Daniel, Shoeb, Mohamed R, Rosenzweig, Sergio D, Boztug, Kaan. . Identification of germline monoallelic mutations in IKZF2 in patients with immune dysregulation. In Blood advances, 6, 2444-2451. doi:10.1182/bloodadvances.2021006367. https://pubmed.ncbi.nlm.nih.gov/34920454/
5. Park, Sun-Mi, Cho, Hyunwoo, Thornton, Angela M, Leslie, Christina, Kharas, Michael G. 2018. IKZF2 Drives Leukemia Stem Cell Self-Renewal and Inhibits Myeloid Differentiation. In Cell stem cell, 24, 153-165.e7. doi:10.1016/j.stem.2018.10.016. https://pubmed.ncbi.nlm.nih.gov/30472158/
6. Xie, Shihao, Wei, Haixia, Peng, Anping, Tian, Xu, Huang, Jun. 2021. Ikzf2 Regulates the Development of ICOS+ Th Cells to Mediate Immune Response in the Spleen of S. japonicum-Infected C57BL/6 Mice. In Frontiers in immunology, 12, 687919. doi:10.3389/fimmu.2021.687919. https://pubmed.ncbi.nlm.nih.gov/34475870/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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