C57BL/6JCya-Chrna9em1flox/Cya
Common Name
Chrna9-flox
Product ID
S-CKO-07442
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-231252-Chrna9-B6J-VA
When using this mouse strain in a publication, please cite “Chrna9-flox Mouse (Catalog S-CKO-07442) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Chrna9-flox
Strain ID
CKOCMP-231252-Chrna9-B6J-VA
Gene Name
Product ID
S-CKO-07442
Gene Alias
Acra9, Gm8311, EG666827, 2410015I05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031108
NCBI RefSeq
NM_001081104
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Chrna9, encoding the nicotinic acetylcholine receptor (nAChR) subunit alpha-9, is a crucial gene. nAChR-mediated signaling pathways, in which Chrna9 is involved, affect the proliferation and differentiation of pluripotent stem cells. It is also associated with the JAK/STAT pathway in glioma [1,2].
Mouse models have been instrumental in understanding Chrna9. In α9-KO mice (lacking the Chrna9 gene), hearing thresholds are normal, but more complex auditory functions like frequency selectivity and sound localization are altered. They also show a reduced capacity to suppress auditory distractors during visual selective attention [4]. In α9α10KO mice (deficient in both alpha9 and alpha10 receptor subunits), there are abnormal auditory processing features despite grossly normal auditory function in quiet and noise, such as larger ABR amplitudes and hyperreactive startles [3].
In conclusion, Chrna9 is vital for the proper function of nAChR-mediated signaling, influencing processes like stem cell regulation and auditory function. Gene-knockout mouse models, especially those targeting Chrna9, have significantly contributed to understanding its role in auditory-related disorders, potentially guiding future research and therapeutic strategies in this area.
References：
1. Yazawa, Takashi, Imamichi, Yoshitaka, Kitano, Takeshi, Umezawa, Akihiro, Uwada, Junsuke. 2023. Expression of Chrna9 is regulated by Tbx3 in undifferentiated pluripotent stem cells. In Scientific reports, 13, 1611. doi:10.1038/s41598-023-28814-7. https://pubmed.ncbi.nlm.nih.gov/36709241/
2. Ma, Xiaoshan, Geng, Ren, Zhao, Yao, Zhao, Liyan, Li, Yunqian. 2024. CHRNA9 as a New Prognostic Marker and Potential Therapeutic Target in Glioma. In Journal of Cancer, 15, 2095-2109. doi:10.7150/jca.92080. https://pubmed.ncbi.nlm.nih.gov/38495483/
3. Mondul, Jane A, Burke, Kali, Morley, Barbara, Lauer, Amanda M. . Alpha9alpha10 knockout mice show altered physiological and behavioral responses to signals in masking noise. In The Journal of the Acoustical Society of America, 155, 3183-3194. doi:10.1121/10.0025985. https://pubmed.ncbi.nlm.nih.gov/38738939/
4. Munoz, Felipe, Vicencio-Jimenez, Sergio, Jorratt, Pascal, Delano, Paul H, Terreros, Gonzalo. 2022. Corticofugal and Brainstem Functions Associated With Medial Olivocochlear Cholinergic Transmission. In Frontiers in neuroscience, 16, 866161. doi:10.3389/fnins.2022.866161. https://pubmed.ncbi.nlm.nih.gov/35573302/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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