C57BL/6JCya-Ffar1em1flox/Cya
Common Name
Ffar1-flox
Product ID
S-CKO-07624
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-233081-Ffar1-B6J-VA
When using this mouse strain in a publication, please cite “Ffar1-flox Mouse (Catalog S-CKO-07624) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ffar1-flox
Strain ID
CKOCMP-233081-Ffar1-B6J-VA
Gene Name
Product ID
S-CKO-07624
Gene Alias
Gpr40
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000052700
NCBI RefSeq
NM_194057
Target Region
Exon 1
Size of Effective Region
~2.4 kb
Overview of Gene Research
Ffar1, also known as GPR40, is a G-protein-coupled receptor for medium-to long-chain free fatty acids. It functions to enhance glucose-stimulated insulin secretion and release of incretin hormones in response to circulating free fatty acids. This makes it a potential target for treating type 2 diabetes mellitus (T2DM) as its activation can regulate glucose levels through glucose-stimulated insulin secretion and incretin production [3,4,5]. It is also involved in many physio-pathological processes [1].
FFAR1-deficient mice were found to exhibit stronger inflammatory and peripheral neuropathic pain-like behavior as well as depressive-like behavior. The findings suggest that FFAR1 indirectly regulates dopamine release by promoting serotonin release, indicating its possible involvement in emotional behaviors [2].
In conclusion, Ffar1 is crucial for glucose-related metabolic regulation and may play a role in emotional behaviors and pain-related responses as revealed by gene-knockout mouse models. Targeting Ffar1 with positive modulators represents an attractive pharmacological approach for T2DM treatment due to its lack of hypoglycaemic side-effects, and may also have potential in alleviating organ inflammation, fibrosis, and treating CNS disorders [1].
References：
1. Governa, Paolo, Caroleo, Maria Cristina, Carullo, Gabriele, Cione, Erika, Manetti, Fabrizio. 2021. FFAR1/GPR40: One target, different binding sites, many agonists, no drugs, but a continuous and unprofitable tug-of-war between ligand lipophilicity, activity, and toxicity. In Bioorganic & medicinal chemistry letters, 41, 127969. doi:10.1016/j.bmcl.2021.127969. https://pubmed.ncbi.nlm.nih.gov/33771587/
2. Kurihara, Takashi. . [Possible involvement of FFAR1 signaling in mouse emotional behaviors through the regulation of brain monoamine releases]. In Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 158, 454-459. doi:10.1254/fpj.23054. https://pubmed.ncbi.nlm.nih.gov/37914322/
3. Paul, Abhik, Nahar, Sourin, Nahata, Pankaj, Karmakar, Sanmoy, Maity, Tapan Kumar. 2023. Synthetic GPR40/FFAR1 agonists: An exhaustive survey on the most recent chemical classes and their structure-activity relationships. In European journal of medicinal chemistry, 264, 115990. doi:10.1016/j.ejmech.2023.115990. https://pubmed.ncbi.nlm.nih.gov/38039791/
4. Li, Zheng, Zhou, Zongtao, Zhang, Luyong. 2019. Current status of GPR40/FFAR1 modulators in medicinal chemistry (2016-2019): a patent review. In Expert opinion on therapeutic patents, 30, 27-38. doi:10.1080/13543776.2020.1698546. https://pubmed.ncbi.nlm.nih.gov/31771391/
5. Li, Zheng, Xu, Xue, Huang, Wenlong, Qian, Hai. 2017. Free Fatty Acid Receptor 1 (FFAR1) as an Emerging Therapeutic Target for Type 2 Diabetes Mellitus: Recent Progress and Prevailing Challenges. In Medicinal research reviews, 38, 381-425. doi:10.1002/med.21441. https://pubmed.ncbi.nlm.nih.gov/28328012/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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