C57BL/6JCya-Edc4em1flox/Cya
Common Name:
Edc4-flox
Product ID:
S-CKO-07757
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Edc4-flox
Strain ID
CKOCMP-234699-Edc4-B6J-VA
Gene Name
Product ID
S-CKO-07757
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Edc4em1flox/Cya mice (Catalog S-CKO-07757) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040254
NCBI RefSeq
NM_001301100
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Edc4, known as enhancer of mRNA-decapping protein 4, is a key scaffold component in mRNA metabolism. It is involved in the deadenylation-dependent mRNA decapping and decay pathway, a major cytoplasmic mRNA turnover mechanism in eukaryotes [1]. It interacts with proteins like the decapping enzyme DCP2 and the 5'-3' exonuclease XRN1, and is also associated with processing bodies (P-bodies), which are ribonucleoprotein granules related to mRNA decay and storage [1,2,3].
Disrupting the EDC4-XRN1 interaction or altering their stoichiometry inhibits mRNA decapping, leading to the stabilization of microRNA-targeted mRNAs in a translationally repressed state. This also results in larger P-bodies that prevent mRNA decapping [1]. In Parkinson's disease, pathological accumulation of α-synuclein leads to aberrant interaction with Edc4, disrupting mRNA decay kinetics in relevant pathways [2]. Silencing EDC4 can activate hallmarks of epithelial-mesenchymal transition (EMT) in cancer cells, enhancing cell migration and metastasis, as P-bodies can repress the translation of the EMT driver gene HMGA2 [4]. EDC4 also interacts with CoA synthase, regulating its dephospho-CoA kinase activity [5]. Additionally, EDC4 is part of the BRCA1-BRIP1-TOPBP1 complex, playing a role in homologous recombination for DNA repair, and its deficiency leads to genome instability and hypersensitivity to certain drugs [6].
In summary, Edc4 has diverse functions in mRNA metabolism, P-body regulation, and DNA repair. Its disruption is associated with diseases like Parkinson's disease and cancer, highlighting its importance in understanding disease mechanisms. Studies on Edc4, especially through loss-of-function models, contribute to revealing its role in these biological processes and disease conditions.
References:
1. Brothers, William R, Ali, Farah, Kajjo, Sam, Fabian, Marc R. 2023. The EDC4-XRN1 interaction controls P-body dynamics to link mRNA decapping with decay. In The EMBO journal, 42, e113933. doi:10.15252/embj.2023113933. https://pubmed.ncbi.nlm.nih.gov/37621215/
2. Hallacli, Erinc, Kayatekin, Can, Nazeen, Sumaiya, Chung, Chee Yeun, Khurana, Vikram. . The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability. In Cell, 185, 2035-2056.e33. doi:10.1016/j.cell.2022.05.008. https://pubmed.ncbi.nlm.nih.gov/35688132/
3. Bloch, Donald B, Sinow, Claire O, Sauer, Andrew J, Corman, Benjamin H P. 2023. Assembly and regulation of the mammalian mRNA processing body. In PloS one, 18, e0282496. doi:10.1371/journal.pone.0282496. https://pubmed.ncbi.nlm.nih.gov/36877681/
4. Fang, Liang, Zhang, Li, Wang, Mengran, Hu, Yuhui, Chen, Wei. . Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition. In Cancer research, 84, 659-674. doi:10.1158/0008-5472.CAN-23-1693. https://pubmed.ncbi.nlm.nih.gov/38190710/
5. Gudkova, Daria, Panasyuk, Ganna, Nemazanyy, Ivan, Filonenko, Valeriy, Gout, Ivan. 2012. EDC4 interacts with and regulates the dephospho-CoA kinase activity of CoA synthase. In FEBS letters, 586, 3590-5. doi:10.1016/j.febslet.2012.08.033. https://pubmed.ncbi.nlm.nih.gov/22982864/
6. Hernández, Gonzalo, Ramírez, María José, Minguillón, Jordi, Pujana, Miquel Àngel, Surrallés, Jordi. 2018. Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. In Nature communications, 9, 967. doi:10.1038/s41467-018-03433-3. https://pubmed.ncbi.nlm.nih.gov/29511213/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen