C57BL/6JCya-Npffr1em1flox/Cya
Common Name
Npffr1-flox
Product ID
S-CKO-07922
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-237362-Npffr1-B6J-VA
When using this mouse strain in a publication, please cite “Npffr1-flox Mouse (Catalog S-CKO-07922) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Npffr1-flox
Strain ID
CKOCMP-237362-Npffr1-B6J-VA
Gene Name
Product ID
S-CKO-07922
Gene Alias
Gm236, NPFF1, Gpr147, NPFF1R, OT7T022
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020287
NCBI RefSeq
NM_001177511
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Overview of Gene Research
Npffr1, also named GPR147, is the receptor for gonadotropin-inhibitory hormone (GnIH) and can bind neuropeptide FF (NPFF) as well. It is a seven-transmembrane G protein-coupled receptor that inhibits cAMP production. Npffr1 is involved in multiple physiological processes, including the regulation of gonadotropin secretion, nociception, and reproductive function, and is part of the RF-amide peptide system [1,4,5].
In a male mouse model of acute fentanyl-induced analgesia and secondary hyperalgesia, npffr1-/-mice showed a marked reversal of fentanyl-induced hyperalgesia, while the blockade of spinal Npffr1 did not enhance fentanyl-induced analgesia as effectively as the blockade of spinal NPFFR2. This indicates that supraspinal Npffr1 plays a role in modulating fentanyl-induced hyperalgesia [2]. In female rats, the unselective Npffr1 antagonist RF9 and the selective antagonist GJ14 affected the PRL secretion induced by Kp and RFRP-3. GJ14 also blunted the estradiol-induced PRL surge, suggesting that Npffr1 is involved in the control of PRL secretion [3].
In conclusion, Npffr1 is essential in regulating gonadotropin and prolactin secretion, as well as modulating opioid-induced hyperalgesia. Gene knockout mouse models have been valuable in revealing its role in these physiological and pain-related processes, providing insights into potential therapeutic targets for conditions related to abnormal hormone regulation and pain management.
References：
1. Ubuka, Takayoshi, Tsutsui, Kazuyoshi. 2014. Evolution of gonadotropin-inhibitory hormone receptor and its ligand. In General and comparative endocrinology, 209, 148-61. doi:10.1016/j.ygcen.2014.09.002. https://pubmed.ncbi.nlm.nih.gov/25220854/
2. Chen, Dan, Zhang, Mengna, Zhang, Qinqin, Xu, Biao, Fang, Quan. 2024. The blockade of neuropeptide FF receptor 1 and 2 differentially contributed to the modulating effects on fentanyl-induced analgesia and hyperalgesia in mice. In European journal of pharmacology, 969, 176457. doi:10.1016/j.ejphar.2024.176457. https://pubmed.ncbi.nlm.nih.gov/38395375/
3. Aquino, Nayara S S, Mansano, Naira S, Vieira, Fernanda A S, Reis, Adelina M, Szawka, Raphael E. . RFamide-related Peptide 3 Signaling via Neuropeptide FF Receptor Stimulates Prolactin Secretion in Female Rats. In Endocrinology, 164, . doi:10.1210/endocr/bqad102. https://pubmed.ncbi.nlm.nih.gov/37403228/
4. Kim, J S. . What's in a Name? Roles of RFamide-Related Peptides Beyond Gonadotrophin Inhibition. In Journal of neuroendocrinology, 28, . doi:10.1111/jne.12407. https://pubmed.ncbi.nlm.nih.gov/27369805/
5. Ayachi, Safia, Simonin, Frédéric. 2014. Involvement of Mammalian RF-Amide Peptides and Their Receptors in the Modulation of Nociception in Rodents. In Frontiers in endocrinology, 5, 158. doi:10.3389/fendo.2014.00158. https://pubmed.ncbi.nlm.nih.gov/25324831/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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