C57BL/6JCya-Mex3dem1flox/Cya
Common Name:
Mex3d-flox
Product ID:
S-CKO-07925
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mex3d-flox
Strain ID
CKOCMP-237400-Mex3d-B6J-VA
Gene Name
Product ID
S-CKO-07925
Gene Alias
Rkhd1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mex3dem1flox/Cya mice (Catalog S-CKO-07925) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105350
NCBI RefSeq
NM_198615
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
MEX3D, a member of the MEX3 (muscle excess 3) family, is an RNA-binding protein. It is involved in post-transcriptional regulation of biological processes, potentially participating in various pathways relevant to diseases [3].
In prostate cancer, MEX3D is highly upregulated by loss of PTEN and/or expression of the TMPRSS2/ERG fusion gene. It drives phenotypes such as soft-agar colony formation, invasion, and tumor formation in vivo, with TCF3 as a major target [1].
In cervical carcinoma, MEX3D is upregulated by HPV16 E7. Knocking down its expression reduces cell proliferation, inhibits colony formation, promotes apoptosis, and reduces tumor growth in mouse xenograft models. MEX3D acts as an RBP to destabilize TSC22D1 mRNA [2].
In non-small-cell lung cancer (NSCLC), overexpression of MEX3D is associated with significantly worse overall survival in patients with LUAD and LUSC, and is correlated with immune cell infiltration [3].
In conclusion, MEX3D is an important RNA-binding protein involved in post-transcriptional regulation. Its dysregulation contributes to the development of prostate cancer, cervical carcinoma, and NSCLC. Functional studies, especially those using in vivo models, have revealed its oncogenic roles in these cancer types, providing potential targets for therapeutic intervention.
References：
1. Shao, Longjiang, Wang, Jianghua, Karatas, Omer, Ittmann, Michael. 2021. MEX3D is an oncogenic driver in prostate cancer. In The Prostate, 81, 1202-1213. doi:10.1002/pros.24216. https://pubmed.ncbi.nlm.nih.gov/34455614/
2. Zheng, Zhi, Chen, Xiaojing, Cai, Xiaoyun, Xu, Junfen, Cheng, Xiaodong. 2022. RNA-binding protein MEX3D promotes cervical carcinoma tumorigenesis by destabilizing TSC22D1 mRNA. In Cell death discovery, 8, 250. doi:10.1038/s41420-022-01049-7. https://pubmed.ncbi.nlm.nih.gov/35513372/
3. Zhang, Ming, Cao, Linfeng, Hou, Gouxin, Lv, Xiaodong, Deng, Jingjing. 2022. Investigation of the Potential Correlation Between RNA-Binding Proteins in the Evolutionarily Conserved MEX3 Family and Non-small-Cell Lung Cancer. In Molecular biotechnology, 65, 1263-1274. doi:10.1007/s12033-022-00638-2. https://pubmed.ncbi.nlm.nih.gov/36507941/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen