C57BL/6JCya-Exd1em1flox/Cya
Common Name:
Exd1-flox
Product ID:
S-CKO-08386
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Exd1-flox
Strain ID
CKOCMP-241624-Exd1-B6J-VA
Gene Name
Product ID
S-CKO-08386
Gene Alias
4932702D22Rik; Exdl1; mExd1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Exd1em1flox/Cya mice (Catalog S-CKO-08386) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060009
NCBI RefSeq
NM_172857
Target Region
Exon 7
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Exd1, short for Exonuclease domain-containing 1, is associated with the biogenesis of PIWI-interacting RNAs (piRNAs) in animal germ cells. PiRNAs play a crucial role in restricting transposon activity to ensure fertility. EXD1 functions in the PIWI-piRNA pathway, which is vital for maintaining the integrity of the germline genome [1,2,3].
In mouse models, loss-of-function experiments of Exd1 have provided insights into its role. Exd1 mutants show reduced MILI-triggered piRNA biogenesis. In the sensitized Exd1 mutant (Exd1-/-;Tdrd12 + /-), decreased MIWI2 piRNA levels lead to the de-repression of LINE1 retrotransposons, ultimately resulting in infertility. This indicates that EXD1 enhances MIWI2 piRNA biogenesis through its interaction with TDRD12 [3].
In conclusion, Exd1 is essential for the biogenesis of piRNAs in the mouse male germline, especially in the context of MIWI2 piRNA biogenesis. The study of Exd1 knockout mouse models has revealed its significance in maintaining the proper regulation of transposons and ensuring male fertility, contributing to our understanding of germline-related diseases and infertility mechanisms.
References：
1. Olotu, Opeyemi, Dowling, Mark, Homolka, David, Pillai, Ramesh S, Kotaja, Noora. 2023. Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes. In Andrology, 11, 710-723. doi:10.1111/andr.13361. https://pubmed.ncbi.nlm.nih.gov/36624638/
2. Yang, Zhaolin, Chen, Kuan-Ming, Pandey, Radha Raman, McCarthy, Andrew A, Pillai, Ramesh S. 2015. PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway. In Molecular cell, 61, 138-52. doi:10.1016/j.molcel.2015.11.009. https://pubmed.ncbi.nlm.nih.gov/26669262/
3. Pandey, Radha Raman, Homolka, David, Olotu, Opeyemi, Kotaja, Noora, Pillai, Ramesh S. . Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12. In Cell reports, 24, 3423-3432.e4. doi:10.1016/j.celrep.2018.08.087. https://pubmed.ncbi.nlm.nih.gov/30257204/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen