C57BL/6JCya-Tarm1em1flox/Cya
Common Name:
Tarm1-flox
Product ID:
S-CKO-08655
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tarm1-flox
Strain ID
CKOCMP-245126-Tarm1-B6J-VA
Gene Name
Product ID
S-CKO-08655
Gene Alias
9930022N03Rik; Gm9904; OLT-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tarm1em1flox/Cya mice (Catalog S-CKO-08655) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000203821
NCBI RefSeq
NM_177363
Target Region
Exon 2~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
TARM1, short for T cell-interacting, activating receptor on myeloid cells 1, is a member of the leukocyte immunoglobulin-like receptor family. It associates with FcRγ and is involved in stimulating macrophages and neutrophils. TARM1 participates in pathways related to inflammation, such as NF-κB pathway, and plays a role in immune-related biological processes [3].
In collagen-induced arthritis (CIA) mouse models, Tarm1-/-mice show suppressed development of CIA, with impaired T cell priming against type 2 collagen and antigen-presenting ability of GM-CSF-induced dendritic cells, indicating TARM1's role in promoting arthritis development [1].
In a mouse colitis model, knockdown of Tarm1 in RAW264.7 cells inhibits lipopolysaccharide (LPS)-induced M1 polarization of macrophages and attenuates intestinal barrier damage [2].
In Mycobacterium tuberculosis infection, TARM-1 knockdown in macrophages reduces pro-inflammatory cytokine expression, HLA-DR and costimulatory molecules, and TARM-1 blocking peptide in mouse models increases bacterial load and lung pathology, suggesting its importance in macrophage activation and Th1 response in this infection [4].
In conclusion, TARM1 is an important factor in the immune system, playing roles in diseases like arthritis, colitis, and tuberculosis. Gene knockout models in mice have been crucial in revealing its functions in these disease-related biological processes, highlighting its potential as a therapeutic target for autoimmune and infectious diseases.
References：
1. Yabe, Rikio, Chung, Soo-Hyun, Murayama, Masanori A, Saijo, Shinobu, Iwakura, Yoichiro. 2021. TARM1 contributes to development of arthritis by activating dendritic cells through recognition of collagens. In Nature communications, 12, 94. doi:10.1038/s41467-020-20307-9. https://pubmed.ncbi.nlm.nih.gov/33397982/
2. Zhang, Kun, Xu, Lingfen, Guo, Jing. 2024. Tarm1 may affect colitis by regulating macrophage M1 polarization in a mouse colitis model. In Pediatric research, , . doi:10.1038/s41390-024-03640-3. https://pubmed.ncbi.nlm.nih.gov/39487321/
3. Radjabova, Valeria, Mastroeni, Piero, Skjødt, Karsten, Trowsdale, John, Barrow, Alexander David. 2015. TARM1 Is a Novel Leukocyte Receptor Complex-Encoded ITAM Receptor That Costimulates Proinflammatory Cytokine Secretion by Macrophages and Neutrophils. In Journal of immunology (Baltimore, Md. : 1950), 195, 3149-59. doi:10.4049/jimmunol.1401847. https://pubmed.ncbi.nlm.nih.gov/26311901/
4. Li, Xingyu, Wang, Manni, Ming, Siqi, Gong, Sitang, Wu, Yongjian. 2021. TARM-1 Is Critical for Macrophage Activation and Th1 Response in Mycobacterium tuberculosis Infection. In Journal of immunology (Baltimore, Md. : 1950), 207, 234-243. doi:10.4049/jimmunol.2001037. https://pubmed.ncbi.nlm.nih.gov/34183366/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen