C57BL/6JCya-Chodlem1flox/Cya
Common Name:
Chodl-flox
Product ID:
S-CKO-08711
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chodl-flox
Strain ID
CKOCMP-246048-Chodl-B6J-VA
Gene Name
Product ID
S-CKO-08711
Gene Alias
3110074E07Rik; MT75; PRED12
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chodlem1flox/Cya mice (Catalog S-CKO-08711) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023568
NCBI RefSeq
NM_139134
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Chodl, or chondrolectin, is a gene encoding a type I transmembrane protein with a C-type lectin carbohydrate recognition domain in its extracellular portion. It is predominantly expressed in muscle cells, such as skeletal muscle, and is also present in testis, brain, and lung in adult mice [4]. Chodl plays roles in processes like motor axon extension, synaptogenesis, and may be involved in pathways related to cell-cell interactions [5]. Gene knockout models can be valuable for studying its functions.
In cancer research, Chodl shows different patterns. In colorectal cancer, its promoter hypermethylation inactivates Chodl, promoting carcinogenesis, and high Chodl expression predicts improved survival [1]. In hepatocellular carcinoma, its expression is decreased, but overexpression in SMMC7721 cells increases cell migration and invasion [2]. In high-grade serous ovarian cancer, elevated CHODL expression is seen in metastasis tissues, and knockdown reduces cell migration and invasion abilities [3]. In lung cancer, high CHODL protein expression is associated with shorter survival, and targeting it could be an anticancer strategy [6].
In zebrafish, Chodl-deficient models show anatomical and functional defects of the neuromuscular synapse, with motor axon growth and branching impaired, indicating its role in synaptogenesis [5].
In conclusion, Chodl is essential for processes like synaptogenesis in neuromuscular junctions. In cancer, it has complex roles where its expression and methylation status can impact tumorigenesis, metastasis, and patient prognosis. Gene knockout models in zebrafish and potentially mouse models in the future can further clarify its functions in these disease areas.
References:
1. Zhang, Xinyue, Wu, Kaiming, Huang, Yuhua, Li, Xiaoxing, Zhang, Ning. 2020. Promoter Hypermethylation of CHODL Contributes to Carcinogenesis and Indicates Poor Survival in Patients with Early-stage Colorectal Cancer. In Journal of Cancer, 11, 2874-2886. doi:10.7150/jca.38815. https://pubmed.ncbi.nlm.nih.gov/32226505/
2. Huang, Zejian, Zhang, Ning, Li, Wenda, Zhang, Lei, Chen, Yajin. 2016. Expression of CHODL in hepatocellular carcinoma affects invasion and migration of liver cancer cells. In Oncology letters, 13, 715-721. doi:10.3892/ol.2016.5466. https://pubmed.ncbi.nlm.nih.gov/28356950/
3. Luo, Haixia, Wang, Kunyu, Li, Bin. 2024. Integrating single-cell and spatial transcriptomic analysis to unveil heterogeneity in high-grade serous ovarian cancer. In Frontiers in immunology, 15, 1420847. doi:10.3389/fimmu.2024.1420847. https://pubmed.ncbi.nlm.nih.gov/38975339/
4. Weng, Lin, Hübner, Roland, Claessens, An, Van Marck, Eric, Merregaert, Jozef. . Isolation and characterization of chondrolectin (Chodl), a novel C-type lectin predominantly expressed in muscle cells. In Gene, 308, 21-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12711387/
5. Oprişoreanu, Ana-Maria, Smith, Hannah L, Arya, Sukrat, Talbot, Kevin, Becker, Catherina G. . Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. In Cell reports, 29, 1082-1098.e10. doi:10.1016/j.celrep.2019.09.033. https://pubmed.ncbi.nlm.nih.gov/31665626/
6. Masuda, Ken, Takano, Atsushi, Oshita, Hideto, Nakamura, Yusuke, Daigo, Yataro. 2011. Chondrolectin is a novel diagnostic biomarker and a therapeutic target for lung cancer. In Clinical cancer research : an official journal of the American Association for Cancer Research, 17, 7712-22. doi:10.1158/1078-0432.CCR-11-0619. https://pubmed.ncbi.nlm.nih.gov/22016508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen