C57BL/6JCya-Slc27a4em1flox/Cya
Common Name:
Slc27a4-flox
Product ID:
S-CKO-09628
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Slc27a4-flox
Strain ID
CKOCMP-26569-Slc27a4-B6J-VA
Gene Name
Product ID
S-CKO-09628
Gene Alias
ACSVL4; FATP4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc27a4em1flox/Cya mice (Catalog S-CKO-09628) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080065
NCBI RefSeq
NM_011989
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Slc27a4, a member of the solute carrier family 27, is crucial for fatty acid synthesis and β -oxidation. It functions as a fatty acid transporter, facilitating the movement of fatty acids across the cell membrane, which is involved in lipid metabolism pathways, a process of great biological importance in maintaining cellular function [2,3].
In hepatocellular carcinoma (HCC), Slc27a4 is significantly overexpressed, especially in the fatty-acids-enriched microenvironment. It promotes the selective uptake of mono-unsaturated fatty acids (MUFAs), leading to increased levels of MUFA-containing phosphatidylcholine and phosphatidylethanolamine in HCC cells, thus conferring resistance to lipid peroxidation and ferroptosis. Silencing Slc27a4 enhances the sensitivity of HCC to sorafenib treatment both in vitro and in vivo [1].
In mice, hepatocyte-specific deletion of Slc27a4 ameliorates non-alcoholic fatty liver disease (NAFLD) by suppressing phosphatidylcholine-mediated PXR activation, while its overexpression in the liver exacerbates diet-induced liver steatosis, inflammation, and fibrosis [2].
In conclusion, Slc27a4 plays a vital role in lipid-related biological processes. Gene-knockout and conditional-knockout mouse models have revealed its significance in diseases like HCC and NAFLD. These models provide valuable insights into the role of Slc27a4, potentially guiding the development of new therapeutic strategies for these diseases.
References:
1. Li, Ziwen, Liao, Xinyi, Hu, Yameng, Xie, Chan, Li, Jun. 2023. SLC27A4-mediated selective uptake of mono-unsaturated fatty acids promotes ferroptosis defense in hepatocellular carcinoma. In Free radical biology & medicine, 201, 41-54. doi:10.1016/j.freeradbiomed.2023.03.013. https://pubmed.ncbi.nlm.nih.gov/36924851/
2. Shen, Chuangpeng, Pan, Zhisen, Xie, Wenmin, Gao, Yong, Liu, Changhui. 2024. Hepatocyte-specific SLC27A4 deletion ameliorates nonalcoholic fatty liver disease in mice via suppression of phosphatidylcholine-mediated PXR activation. In Metabolism: clinical and experimental, 162, 156054. doi:10.1016/j.metabol.2024.156054. https://pubmed.ncbi.nlm.nih.gov/39489412/
3. Schwenk, Robert W, Holloway, Graham P, Luiken, Joost J F P, Bonen, Arend, Glatz, Jan F C. 2010. Fatty acid transport across the cell membrane: regulation by fatty acid transporters. In Prostaglandins, leukotrienes, and essential fatty acids, 82, 149-54. doi:10.1016/j.plefa.2010.02.029. https://pubmed.ncbi.nlm.nih.gov/20206486/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen