C57BL/6JCya-Scmh1em1flox/Cya
Common Name
Scmh1-flox
Product ID
S-CKO-10190
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-29871-Scmh1-B6J-VA
When using this mouse strain in a publication, please cite “Scmh1-flox Mouse (Catalog S-CKO-10190) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Scmh1-flox
Strain ID
CKOCMP-29871-Scmh1-B6J-VA
Gene Name
Product ID
S-CKO-10190
Gene Alias
Scml1, Scml3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000000087
NCBI RefSeq
NM_013883
Target Region
Exon 7
Size of Effective Region
~1.4 kb
Overview of Gene Research
Scmh1, as a substoichiometric component of Polycomb-group (PcG) complex 1, has E3 ubiquitin ligase activity for geminin and histone H2A. It regulates geminin stability either directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4, which are direct targets of PcG complex 1-mediated transcriptional silencing in hematopoietic cells [6].
Circular RNA SCMH1 (circSCMH1), a product related to the Scmh1 gene, has shown potential in multiple disease-related studies. In ischemic stroke, circSCMH1 levels are decreased in patients' plasma. Treating rodent and nonhuman primate ischemic stroke models with engineered extracellular vesicles delivering circSCMH1 promotes functional recovery, enhances neuronal plasticity, and inhibits glial activation and peripheral immune cell infiltration. It binds to the transcription factor MeCP2, releasing repression of MeCP2 target gene transcription [1]. In addition, circSCMH1 also promotes vascular repair in the peri-infarct cortex of male mice and monkeys after stroke by regulating FTO-dependent m6A modification of Plpp3 [2], and suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke [3]. In other diseases, circSCMH1 in high-glucose-treated ARPE-19 cells promotes diabetes-induced retinal epithelial-mesenchymal transition by regulating the miR-200a-3p/ZEB1 signaling axis [4], and in oral squamous cell carcinoma, circ-SCMH1 contributes to cis-dichlorodiammineplatinum (DDP) resistance via the circ-SCMH1/miR-338-3p/LIN28B axis [5]. Also, in Parkinson's disease, hsa-SCMH1_0001, a circular RNA, may be involved in the pathogenesis [7], and in traumatic brain injury, exosomes from adipose-derived stem cells delivering circ-Scmh1 can promote microglial M2 polarization and ameliorate hippocampal nerve damage [8].
In summary, Scmh1 and its related circular RNAs play essential roles in various biological processes and disease conditions. The study of Scmh1-related circular RNAs in different disease models, especially in ischemic stroke, diabetes-induced retinopathy, oral squamous cell carcinoma, Parkinson's disease, and traumatic brain injury, has provided insights into disease mechanisms and potential therapeutic targets.
References：
1. Yang, Li, Han, Bing, Zhang, Zhiting, Wang, Jianhong, Yao, Honghong. 2020. Extracellular Vesicle-Mediated Delivery of Circular RNA SCMH1 Promotes Functional Recovery in Rodent and Nonhuman Primate Ischemic Stroke Models. In Circulation, 142, 556-574. doi:10.1161/CIRCULATIONAHA.120.045765. https://pubmed.ncbi.nlm.nih.gov/32441115/
2. Li, Bin, Xi, Wen, Bai, Ying, Han, Bing, Yao, Honghong. 2023. FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke. In Nature communications, 14, 489. doi:10.1038/s41467-023-36008-y. https://pubmed.ncbi.nlm.nih.gov/36717587/
3. Wang, Yu, Bai, Ying, Cai, Yang, Han, Bing, Yao, Honghong. 2024. Circular RNA SCMH1 suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke. In Theranostics, 14, 7292-7308. doi:10.7150/thno.99323. https://pubmed.ncbi.nlm.nih.gov/39659575/
4. He, Ye, Hou, Xiang-Tao, Guan, Yu-Xin, Chen, Yao. 2022. CircRNA SCMH1 regulates the miR-200a-3p/ZEB1 signaling axis to promote diabetes-induced retinal epithelial-mesenchymal transition. In Experimental eye research, 224, 109264. doi:10.1016/j.exer.2022.109264. https://pubmed.ncbi.nlm.nih.gov/36162459/
5. Qiu, Feng, Qiao, Bin, Zhang, Nan, Zhang, Shanfeng, Qiu, Weiliu. 2021. Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B. In Cancer cell international, 21, 412. doi:10.1186/s12935-021-02110-8. https://pubmed.ncbi.nlm.nih.gov/34353342/
6. Yasunaga, Shin'ichiro, Ohtsubo, Motoaki, Ohno, Yoshinori, Miyoshi, Jun, Takihara, Yoshihiro. 2012. Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4. In Molecular and cellular biology, 33, 644-60. doi:10.1128/MCB.00974-12. https://pubmed.ncbi.nlm.nih.gov/23207902/
7. Wang, Qiao, Wang, Huizhi, Zhao, Xuemin, Cai, Guoen, Meng, Fangang. 2023. Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease. In CNS neuroscience & therapeutics, 30, e14435. doi:10.1111/cns.14435. https://pubmed.ncbi.nlm.nih.gov/37664885/
8. Chen, Songyu, Wang, Xuewei, Qian, Zhouqi, Li, Lei, Gao, Liang. 2023. Exosomes from ADSCs ameliorate nerve damage in the hippocampus caused by post traumatic brain injury via the delivery of circ-Scmh1 promoting microglial M2 polarization. In Injury, 54, 110927. doi:10.1016/j.injury.2023.110927. https://pubmed.ncbi.nlm.nih.gov/37544863/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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