C57BL/6JCya-Batf3em1flox/Cya
Common Name:
Batf3-flox
Product ID:
S-CKO-10925
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Batf3-flox
Strain ID
CKOCMP-381319-Batf3-B6J-VA
Gene Name
Product ID
S-CKO-10925
Gene Alias
9130211I03Rik; Snft
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Batf3em1flox/Cya mice (Catalog S-CKO-10925) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027943
NCBI RefSeq
NM_030060
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Batf3, also known as basic leucine zipper transcription factor ATF-like 3, is a transcription factor. It is essential in the development of mucosal conventional DCs type 1 (cDC1) [2]. It is involved in multiple biological processes related to the immune system, including T-cell-mediated immunity and maintaining intestinal epithelial homeostasis. Genetic models, such as gene knockout mouse models, are valuable tools for studying its functions.
In Batf3 -/- mice, the development of CD8α+ dendritic cells was ablated. These mice were defective in cross-presentation, lacked virus-specific CD8+ T cell responses to West Nile virus, and had impaired rejection of highly immunogenic syngeneic tumors, suggesting an important role for CD8α+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection [1]. Batf3 -/- mice also developed metabolic syndrome, had altered localization of tight junction proteins in intestinal epithelial cells leading to increased intestinal permeability, and displayed microbial dysbiosis [2]. Additionally, T cells lacking Batf3 showed normal expansion and differentiation but an aggravated contraction and a diminished memory response, while overexpression of BATF3 in CD8+ T cells promoted their survival and transition to memory [3].
In conclusion, Batf3 plays crucial roles in the immune system, including in T-cell-mediated immunity, virus responses, tumor rejection, and maintaining intestinal epithelial homeostasis. The Batf3 knockout mouse models have significantly contributed to understanding its functions in these disease-related areas such as viral infections, tumor immunity, and metabolic syndrome.
References:
1. Hildner, Kai, Edelson, Brian T, Purtha, Whitney E, Murphy, Theresa L, Murphy, Kenneth M. . Batf3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity. In Science (New York, N.Y.), 322, 1097-100. doi:10.1126/science.1164206. https://pubmed.ncbi.nlm.nih.gov/19008445/
2. Hamade, Hussein, Stamps, Jasmine T, Stamps, Dalton T, Targan, Stephan R, Michelsen, Kathrin S. 2022. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. In Frontiers in immunology, 13, 841065. doi:10.3389/fimmu.2022.841065. https://pubmed.ncbi.nlm.nih.gov/35812447/
3. Ataide, Marco A, Komander, Karl, Knöpper, Konrad, Vaeth, Martin, Kastenmüller, Wolfgang. 2020. BATF3 programs CD8+ T cell memory. In Nature immunology, 21, 1397-1407. doi:10.1038/s41590-020-0786-2. https://pubmed.ncbi.nlm.nih.gov/32989328/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen