C57BL/6JCya-Mir140em1flox/Cya
Common Name
Mir140-flox
Product ID
S-CKO-11101
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-387158-Mir140-B6J-VA
When using this mouse strain in a publication, please cite “Mir140-flox Mouse (Catalog S-CKO-11101) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mir140-flox
Strain ID
CKOCMP-387158-Mir140-B6J-VA
Gene Name
Product ID
S-CKO-11101
Gene Alias
Mirn140, mir-140, mmu-mir-140
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000083505
NCBI RefSeq
NR_029553
Target Region
Exon 1
Size of Effective Region
~1.5 kb
Overview of Gene Research
Mir140 is a microRNA that plays significant roles in various biological processes. It is involved in cartilage development [3,4,6]. In skeletal progenitor cells and chondrocytes, it regulates aspects of cellular function, thus contributing to skeletal development [4]. Dysregulation of Mir140 can lead to skeletal dysplasias, highlighting its importance in normal skeletal formation [4,5].
In obesity-related bone deterioration, conditional knockout of BMM miR-140 in mice showed resistance to obesity-induced bone deterioration. This indicates that Mir140 in bone marrow macrophages (BMMs) promotes obesity-induced bone deterioration, likely by regulating the differentiation of skeletal stem/progenitor cells (SSPCs) towards adipocytes as it has the function of promoting adipogenesis [1]. In knee osteoarthritis, exosomes derived from human urine-derived stem cells overexpressing miR-140-5p alleviated the condition in a rat model. This was achieved through down-regulation of VEGFA, enhancing chondrocyte proliferation, migration, and extracellular matrix secretion, as well as cartilage regeneration and subchondral bone remodeling [2].
In conclusion, Mir140 is crucial for cartilage development and skeletal formation. Its dysregulation can lead to skeletal dysplasias. In the context of obesity-related bone deterioration and knee osteoarthritis, findings from gene-knockout or over-expression models in mice have revealed its role in disease progression and potential as a therapeutic target. Understanding Mir140's function contributes to the development of treatments for these bone-related diseases.
References：
1. He, Chen, Hu, Chen, He, Wen-Zhen, Lei, Guang-Hua, Li, Chang-Jun. 2024. Macrophage-derived extracellular vesicles regulate skeletal stem/progenitor Cell lineage fate and bone deterioration in obesity. In Bioactive materials, 36, 508-523. doi:10.1016/j.bioactmat.2024.06.035. https://pubmed.ncbi.nlm.nih.gov/39072285/
2. Liu, Yuan, Zeng, Yi, Si, Hai-Bo, Xie, Hui-Qi, Shen, Bin. 2022. Exosomes Derived From Human Urine-Derived Stem Cells Overexpressing miR-140-5p Alleviate Knee Osteoarthritis Through Downregulation of VEGFA in a Rat Model. In The American journal of sports medicine, 50, 1088-1105. doi:10.1177/03635465221073991. https://pubmed.ncbi.nlm.nih.gov/35179989/
3. Tanaka-Watanabe, Yoko, Asahara, Hiroshi. . [Joint and microRNA]. In Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology, 35, 447-54. doi:. https://pubmed.ncbi.nlm.nih.gov/23291479/
4. Shvedova, Maria, Kobayashi, Tatsuya. 2020. MicroRNAs in cartilage development and dysplasia. In Bone, 140, 115564. doi:10.1016/j.bone.2020.115564. https://pubmed.ncbi.nlm.nih.gov/32745689/
5. Goel, Himanshu, Goel, Amy. 2024. MicroRNA and Rare Human Diseases. In Genes, 15, . doi:10.3390/genes15101243. https://pubmed.ncbi.nlm.nih.gov/39457367/
6. Simon, Tommie C, Jeffries, Matlock A. . The Epigenomic Landscape in Osteoarthritis. In Current rheumatology reports, 19, 30. doi:10.1007/s11926-017-0661-9. https://pubmed.ncbi.nlm.nih.gov/28456906/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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