C57BL/6JCya-Tnni3kem1flox/Cya
Common Name:
Tnni3k-flox
Product ID:
S-CKO-11321
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tnni3k-flox
Strain ID
CKOCMP-435766-Tnni3k-B6J-VA
Gene Name
Product ID
S-CKO-11321
Gene Alias
Cark; D830019J24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnni3kem1flox/Cya mice (Catalog S-CKO-11321) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064076
NCBI RefSeq
NM_177066
Target Region
Exon 3~4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Tnni3k, short for troponin I-interacting kinase, is a kinase mainly expressed in the heart [1,2,3]. It has been implicated in multiple cardiac phenotypes and physiological processes. Although its exact associated pathways remain to be fully elucidated, it appears to play a significant role in cardiac-related biological functions, making it an important target for understanding heart-related diseases [1,2]. Genetic models, such as mouse models, have been crucial in studying Tnni3k's functions [4,5].
In mice, Tnni3k has been shown to influence cardiomyocyte S-phase activity and proliferation. Retrospective analysis of single-cell ventricular suspensions in mice determined that Tnni3k definitively impacts both early and final stages of the cell cycle [4]. Additionally, studies on Tnni3k alleles in mice showed that its function is dependent on its kinase activity. Two kinase-domain frameshift mutations in mice led to loss-of-function consequences via nonsense-mediated decay. These findings suggest that Tnni3k may influence heart regenerative capacity in mice and potentially in humans as well [5].
In conclusion, Tnni3k plays essential roles in cardiomyocyte cell-cycle regulation and heart regenerative processes as revealed through mouse-based research. Mouse models, especially those with gene knockout or conditional knockout of Tnni3k, have provided valuable insights into its role in cardiac-related disease conditions such as cardiomyopathy, arrhythmias, and potentially heart failure [1,2,4,5].
References:
1. Pham, Caroline, Muñoz-Martín, Noelia, Lodder, Elisabeth M. 2021. The Diverse Roles of TNNI3K in Cardiac Disease and Potential for Treatment. In International journal of molecular sciences, 22, . doi:10.3390/ijms22126422. https://pubmed.ncbi.nlm.nih.gov/34203974/
2. Milano, Annalisa, Lodder, Elisabeth M, Bezzina, Connie R. 2015. TNNI3K in cardiovascular disease and prospects for therapy. In Journal of molecular and cellular cardiology, 82, 167-73. doi:10.1016/j.yjmcc.2015.03.008. https://pubmed.ncbi.nlm.nih.gov/25787061/
3. Lai, Zhong-Fang. . TNNI3K could be a novel molecular target for the treatment of cardiac diseases. In Recent patents on cardiovascular drug discovery, 4, 203-10. doi:. https://pubmed.ncbi.nlm.nih.gov/19925440/
4. Purdy, Alexandra L, Swift, Samantha K, Sucov, Henry M, Patterson, Michaela. 2023. Tnni3k influences cardiomyocyte S-phase activity and proliferation. In Journal of molecular and cellular cardiology, 183, 22-26. doi:10.1016/j.yjmcc.2023.08.004. https://pubmed.ncbi.nlm.nih.gov/37597489/
5. Gan, Peiheng, Patterson, Michaela, Velasquez, Alexa, Park, Thomas J, Sucov, Henry M. 2019. Tnni3k alleles influence ventricular mononuclear diploid cardiomyocyte frequency. In PLoS genetics, 15, e1008354. doi:10.1371/journal.pgen.1008354. https://pubmed.ncbi.nlm.nih.gov/31589606/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen