C57BL/6JCya-Clec4a4em1flox/Cya
Common Name
Clec4a4-flox
Product ID
S-CKO-11341
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-474145-Clec4a4-B6J-VA
When using this mouse strain in a publication, please cite “Clec4a4-flox Mouse (Catalog S-CKO-11341) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clec4a4-flox
Strain ID
CKOCMP-474145-Clec4a4-B6J-VA
Gene Name
Product ID
S-CKO-11341
Gene Alias
Dcir2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000079379
NCBI RefSeq
NM_001005860
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Overview of Gene Research
Clec4a4, also known as DCIR2 (Dendritic cell immunoreceptor 2), is a C-type lectin receptor. It is mainly expressed by CD8α-DCs and can modulate T cell immunity, playing a role in regulating the function of dendritic cells (DCs) and affecting immune responses [1,3].
In atherosclerosis, Clec4a4 deficiency in Ldlr -/- mice leads to reduced atherosclerotic plaque formation, along with altered lipid metabolism and myeloid immune cell distribution, suggesting a pro-atherosclerotic role of Clec4a4 [1].
In tumor immunity, mClec4A4 (murine Clec4A4) deficiency reduces tumor development due to enhanced antitumor immune responses and an improved immunosuppressive tumor microenvironment, indicating it acts as a negative immune checkpoint regulator [2].
In addition, Clec4a4 -/- CD8α-cDCs show enhanced cytokine production and T-cell priming, and Clec4a4 -/- mice exhibit TLR-mediated hyperinflammation, augmented T-cell responses, and progressive autoimmune pathogenesis upon antigenic immunization, highlighting its role in regulating the magnitude and quality of immune response [3].
In conclusion, Clec4a4 is a key regulator in immune-related processes. Its deficiency in KO mouse models reveals its pro-atherosclerotic role and its function as a negative immune checkpoint regulator in tumor immunity. These findings contribute to understanding the mechanisms of atherosclerosis and tumor development, potentially providing new targets for related disease treatments.
References：
1. Bellini, Rossella, Moregola, Annalisa, Nour, Jasmine, Bonacina, Fabrizia, Norata, Giuseppe Danilo. 2022. Dendritic cell marker Clec4a4 deficiency limits atherosclerosis progression. In Atherosclerosis plus, 51, 8-12. doi:10.1016/j.athplu.2022.12.001. https://pubmed.ncbi.nlm.nih.gov/36969702/
2. Uto, Tomofumi, Fukaya, Tomohiro, Mitoma, Shuya, Hishikawa, Yoshitaka, Sato, Katsuaki. . Clec4A4 Acts as a Negative Immune Checkpoint Regulator to Suppress Antitumor Immunity. In Cancer immunology research, 11, 1266-1279. doi:10.1158/2326-6066.CIR-22-0536. https://pubmed.ncbi.nlm.nih.gov/37432112/
3. Uto, Tomofumi, Fukaya, Tomohiro, Takagi, Hideaki, Malissen, Bernard, Sato, Katsuaki. 2016. Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity. In Nature communications, 7, 11273. doi:10.1038/ncomms11273. https://pubmed.ncbi.nlm.nih.gov/27068492/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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