C57BL/6NCya-Elaneem1flox/Cya
Common Name
Elane-flox
Product ID
S-CKO-11367
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-50701-Elane-B6N-VA
When using this mouse strain in a publication, please cite “Elane-flox Mouse (Catalog S-CKO-11367) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Elane-flox
Strain ID
CKOCMP-50701-Elane-B6N-VA
Gene Name
Product ID
S-CKO-11367
Gene Alias
Ela2, F430011M15Rik, NE
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000046091
NCBI RefSeq
NM_015779
Target Region
Exon 1~3
Size of Effective Region
~1.1 kb
Overview of Gene Research
Elane, encoding neutrophil elastase, is closely associated with neutrophil maturation and functions [2,4,5,6,8]. Neutrophil elastase is involved in leukocyte transmigration to infection sites and clearance of Gram-negative bacteria, but also activates inflammation in chronic airway diseases [7]. It is crucial in innate immune responses and has been linked to multiple disease-related pathways.
Mutations in Elane cause cyclic neutropenia (CyN) and severe congenital neutropenia (SCN), with diverse mutations observed in patients [4]. These mutations can lead to impaired neutrophil differentiation, and patients may be at risk of developing myelodysplasia or acute myeloid leukemia [2,4,5,8]. In gastric cancer, Elane is significantly up-regulated, affecting patient survival and involved in pyroptosis and immune infiltration, potentially serving as a prognostic biomarker [1]. Human neutrophils release catalytically active Elane to selectively kill cancer cells, suggesting its potential as an anti-cancer therapy [3].
In conclusion, Elane is essential for neutrophil-related functions and innate immunity. Research on Elane-associated neutropenia and its role in cancer, through genetic models and patient studies, has provided insights into disease mechanisms. Understanding Elane's functions can offer new perspectives for treating neutropenia, leukemia, and gastric cancer.
References：
1. Cui, Ming, Wang, Xiaowu, Qiao, Haiyan, Wu, Shixi, Shang, Bingbing. 2024. ELANE is a promising prognostic biomarker that mediates pyroptosis in gastric cancer. In Heliyon, 10, e34360. doi:10.1016/j.heliyon.2024.e34360. https://pubmed.ncbi.nlm.nih.gov/39130462/
2. Nasri, Masoud, Ritter, Malte U, Mir, Perihan, Welte, Karl, Skokowa, Julia. 2024. CRISPR-Cas9n-mediated ELANE promoter editing for gene therapy of severe congenital neutropenia. In Molecular therapy : the journal of the American Society of Gene Therapy, 32, 1628-1642. doi:10.1016/j.ymthe.2024.03.037. https://pubmed.ncbi.nlm.nih.gov/38556793/
3. Cui, Chang, Chakraborty, Kasturi, Tang, Xu Anna, Greene, Geoffrey, Becker, Lev. 2021. Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis. In Cell, 184, 3163-3177.e21. doi:10.1016/j.cell.2021.04.016. https://pubmed.ncbi.nlm.nih.gov/33964209/
4. Makaryan, Vahagn, Zeidler, Cornelia, Bolyard, Audrey Anna, Welte, Karl, Dale, David C. . The diversity of mutations and clinical outcomes for ELANE-associated neutropenia. In Current opinion in hematology, 22, 3-11. doi:10.1097/MOH.0000000000000105. https://pubmed.ncbi.nlm.nih.gov/25427142/
5. Skokowa, Julia, Dale, David C, Touw, Ivo P, Zeidler, Cornelia, Welte, Karl. 2017. Severe congenital neutropenias. In Nature reviews. Disease primers, 3, 17032. doi:10.1038/nrdp.2017.32. https://pubmed.ncbi.nlm.nih.gov/28593997/
6. Rao, Shuquan, Yao, Yao, Soares de Brito, Josias, Newburger, Peter E, Bauer, Daniel E. 2021. Dissecting ELANE neutropenia pathogenicity by human HSC gene editing. In Cell stem cell, 28, 833-845.e5. doi:10.1016/j.stem.2020.12.015. https://pubmed.ncbi.nlm.nih.gov/33513358/
7. Voynow, Judith A, Shinbashi, Meagan. 2021. Neutrophil Elastase and Chronic Lung Disease. In Biomolecules, 11, . doi:10.3390/biom11081065. https://pubmed.ncbi.nlm.nih.gov/34439732/
8. Wang, Jing, Zhang, Haitao, Wang, Yu, Liang, Lei, Yang, Zeyu. . Severe congenital neutropenia caused by ELANE gene mutation: A case report and literature review. In Medicine, 101, e31357. doi:10.1097/MD.0000000000031357. https://pubmed.ncbi.nlm.nih.gov/36343040/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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