C57BL/6JCya-Mcrs1em1flox/Cya
Common Name:
Mcrs1-flox
Product ID:
S-CKO-11458
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mcrs1-flox
Strain ID
CKOCMP-51812-Mcrs1-B6J-VA
Gene Name
Product ID
S-CKO-11458
Gene Alias
ICP22BP; MSP58; P78
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcrs1em1flox/Cya mice (Catalog S-CKO-11458) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041190
NCBI RefSeq
NM_016766
Target Region
Exon 7~8
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mcrs1, also known as Microspherule protein 1 or MSP58, is a multifunctional protein involved in various key cellular processes and biological pathways. It is a component of the nonspecific lethal (NSL) complex and the chromatin remodeling INO80 complex, participating in transcriptional regulation during mitosis. It has significant importance in processes like cell cycle regulation, spindle assembly, and embryogenesis [2].
In mouse oocytes, Mcrs1 depletion delays the G2/M transition and disrupts spindle assembly, highlighting its role in cell cycle regulation and spindle organization [2]. Mcrs1 is essential for epiblast development during early mouse embryogenesis; mutant embryos fail to implant due to compromised epiblast lineage [3]. In zebrafish, homozygous mcrs1 mutants show excessive apoptosis, resulting in reduced brain and eye size, and defects in retinal layer formation and melanosome aggregation [5]. In cancer research, high MCRS1 expression in gastric cancer is associated with increased tumor growth, invasiveness, and metastasis, negatively impacting patient survival [4]. In pancreatic cancer, MCRS1 can augment MHC-I expression, sensitizing cancer cells to T cell immunity and enhancing the effectiveness of α -PD-1 therapy [1].
In conclusion, Mcrs1 plays crucial roles in cell cycle regulation, spindle assembly, embryogenesis, and cancer development. Studies using gene knockout (KO) or conditional knockout (CKO) mouse models and other loss-of-function experiments have revealed its significance in these biological processes and disease conditions, providing valuable insights for understanding related mechanisms and potentially developing therapeutic strategies.
References:
1. Li, Xue, Yi, Han, Jin, Zheyu, Liu, Yanfang, Yu, Xiaofei. 2024. MCRS1 sensitizes T cell-dependent immunotherapy by augmenting MHC-I expression in solid tumors. In The Journal of experimental medicine, 221, . doi:10.1084/jem.20240959. https://pubmed.ncbi.nlm.nih.gov/39545935/
2. Ju, Jia-Qian, Pan, Zhen-Nan, Zhang, Kun-Huan, Liu, Jing-Cai, Sun, Shao-Chen. 2023. Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis. In EMBO reports, 24, e56273. doi:10.15252/embr.202256273. https://pubmed.ncbi.nlm.nih.gov/36951681/
3. Cui, Wei, Cheong, Agnes, Wang, Yongsheng, Tremblay, Kimberly D, Mager, Jesse. . MCRS1 is essential for epiblast development during early mouse embryogenesis. In Reproduction (Cambridge, England), 159, 1-13. doi:10.1530/REP-19-0334. https://pubmed.ncbi.nlm.nih.gov/31671403/
4. Wang, Liang-Han, Chang, Chih-Chun, Cheng, Chiao-Yin, Sun, Jen-Tang, Chen, Yen-Lin. 2022. MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer. In Diagnostics (Basel, Switzerland), 12, . doi:10.3390/diagnostics12061502. https://pubmed.ncbi.nlm.nih.gov/35741311/
5. Lee, Si-Hyung, Lee, Mi-Sun, Choi, Tae-Ik, Kim, Cheol-Hee, Kim, Joon. 2016. MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. In Scientific reports, 6, 27284. doi:10.1038/srep27284. https://pubmed.ncbi.nlm.nih.gov/27263857/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen