C57BL/6JCya-Atxn1lem1flox/Cya
Common Name:
Atxn1l-flox
Product ID:
S-CKO-11497
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Atxn1l-flox
Strain ID
CKOCMP-52335-Atxn1l-B6J-VA
Gene Name
Product ID
S-CKO-11497
Gene Alias
Boat; C330011L24Rik; D6Mgi37; D8Ertd587e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atxn1lem1flox/Cya mice (Catalog S-CKO-11497) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093162
NCBI RefSeq
NM_001080930
Target Region
Exon 4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Atxn1l, also known as ataxin 1-like, is a protein-coding gene. It is involved in multiple biological processes. It interacts with CIC (capicua) to form a transcription repressor complex, and this complex plays a role in modulating the sensitivity to MAPK pathway inhibition [1,3,4]. It is also related to the Notch-driven marginal zone B cell development and sepsis progression [2].
In loss-of-function studies, ATXN1L deletion reduces CIC protein, which in turn modulates sensitivity to the MEK1/2 inhibitor trametinib in cancer cells [1]. In B cell-specific Atxn1l-deficient (Atxn1lf/f;Cd19-Cre) mice, Notch signaling is disrupted in marginal zone B cells, leading to inhibited MZB cell development due to ETV4 de-repression and subsequent inhibition of Notch1 and Notch2 transcription. Also, in these mice, humoral immune responses and lipopolysaccharide-induced sepsis progression are attenuated but restored upon Etv4-deletion [2].
In conclusion, Atxn1l is essential for the regulation of multiple biological processes, especially through its interaction with CIC. The Atxn1l-deficient mouse models have revealed its significance in cancer drug sensitivity, B cell development, and sepsis progression, providing valuable insights into the underlying mechanisms of these biological processes and disease conditions.
References:
1. Wang, Belinda, Krall, Elsa Beyer, Aguirre, Andrew James, Root, David Edward, Hahn, William Chun. . ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to MAPK Pathway Inhibition. In Cell reports, 18, 1543-1557. doi:10.1016/j.celrep.2017.01.031. https://pubmed.ncbi.nlm.nih.gov/28178529/
2. Park, Jong Seok, Kang, Minjung, Kim, Han Bit, Kim, Tae-Kyung, Lee, Yoontae. 2024. The capicua-ataxin-1-like complex regulates Notch-driven marginal zone B cell development and sepsis progression. In Nature communications, 15, 10579. doi:10.1038/s41467-024-54803-z. https://pubmed.ncbi.nlm.nih.gov/39632849/
3. Wong, Derek, Lounsbury, Kohl, Lum, Amy, Marra, Marco, Yip, Stephen. 2018. Transcriptomic analysis of CIC and ATXN1L reveal a functional relationship exploited by cancer. In Oncogene, 38, 273-290. doi:10.1038/s41388-018-0427-5. https://pubmed.ncbi.nlm.nih.gov/30093628/
4. Wong, Derek, Sogerer, Lisa, Lee, Samantha S, Marra, Marco A, Yip, Stephen. 2020. TRIM25 promotes Capicua degradation independently of ERK in the absence of ATXN1L. In BMC biology, 18, 154. doi:10.1186/s12915-020-00895-0. https://pubmed.ncbi.nlm.nih.gov/33115448/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen