C57BL/6JCya-Unc93b1em1flox/Cya
Common Name:
Unc93b1-flox
Product ID:
S-CKO-11760
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Unc93b1-flox
Strain ID
CKOCMP-54445-Unc93b1-B6J-VA
Gene Name
Product ID
S-CKO-11760
Gene Alias
Unc-93B1; Unc93b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Unc93b1em1flox/Cya mice (Catalog S-CKO-11760) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000162708
NCBI RefSeq
NM_019449
Target Region
Exon 4~7
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
UNC93B1, the Unc-93 homolog B1, is a transmembrane protein crucial for the trafficking and function of nucleic acid-sensing Toll-like receptors (TLRs) such as TLR3, TLR7, TLR8, and TLR9, which are vital for antiviral immunity. It also plays a role in the cGAS-STING signaling pathway [1,2].
In KO mouse models, Unc93b1-deficient mice show enhanced IFN-β, ISG54, and ISG56 transcriptions, TBK1 phosphorylation, reduced STING degradation, and lower viral replication upon herpes simplex virus-1 (HSV-1) infection. Heterozygous and homozygous mice expressing the orthologous Unc93b1V117L variant develop a spontaneous lupus-like disease and are hyperresponsive to TLR7 stimulation [2,3].
In conclusion, UNC93B1 is essential for regulating TLR-mediated innate immunity and the cGAS-STING signaling pathway. Mouse models with Unc93b1 deficiency or specific variants have revealed its role in antiviral innate immunity and in predisposing to lupus-like diseases, providing insights into the mechanisms of autoimmune diseases and potential therapeutic targets.
References：
1. Wolf, Christine, Lim, Ee Lyn, Mokhtari, Mohammad, Majer, Olivia, Lee-Kirsch, Min Ae. 2024. UNC93B1 variants underlie TLR7-dependent autoimmunity. In Science immunology, 9, eadi9769. doi:10.1126/sciimmunol.adi9769. https://pubmed.ncbi.nlm.nih.gov/38207055/
2. Zhu, Huifang, Zhang, Rongzhao, Yi, Li, Tang, Yan-Dong, Zheng, Chunfu. 2022. UNC93B1 attenuates the cGAS-STING signaling pathway by targeting STING for autophagy-lysosome degradation. In Journal of medical virology, 94, 4490-4501. doi:10.1002/jmv.27860. https://pubmed.ncbi.nlm.nih.gov/35577759/
3. Al-Azab, Mahmoud, Idiiatullina, Elina, Liu, Ziyang, Zhang, Yuxia, Masters, Seth L. 2024. Genetic variants in UNC93B1 predispose to childhood-onset systemic lupus erythematosus. In Nature immunology, 25, 969-980. doi:10.1038/s41590-024-01846-5. https://pubmed.ncbi.nlm.nih.gov/38831104/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen